PerCP/Cy5.5® Anti-CD3 antibody [UCHT1], prediluted (ab157317)
Key features and details
- PerCP/Cy5.5® Mouse monoclonal [UCHT1] to CD3, prediluted
- Suitable for: Flow Cyt, Flow Cyt (Intra)
- Reacts with: Human, Non human primates
- Conjugation: PerCP/Cy5.5®. Ex: 482nm, Em: 690nm
- Isotype: IgG1
Overview
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Product name
PerCP/Cy5.5® Anti-CD3 antibody [UCHT1], prediluted
See all CD3 primary antibodies -
Description
PerCP/Cy5.5® Mouse monoclonal [UCHT1] to CD3, prediluted -
Host species
Mouse -
Conjugation
PerCP/Cy5.5®. Ex: 482nm, Em: 690nm -
Specificity
ab157317 reacts with the epsilon chain of the CD3 complex. -
Tested applications
Suitable for: Flow Cyt, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human, Non human primates -
Immunogen
Tissue, cells or virus corresponding to Human CD3. Human thymocytes followed by Sezary T cells.
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Positive control
- Flow Cytometry: human peripheral blood cells. Flow Cyt (intra): Human blood cells.
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General notes
Purified antibody is conjugated with tandem dye PerCP/Cy5.5® under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: 99% PBS, 0.2% BSA -
Concentration information loading...
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Purity
Size exclusion -
Clonality
Monoclonal -
Clone number
UCHT1 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
- PerCP Anti-CD3 antibody [UCHT1], prediluted (ab106215)
- PE/Cy5® Anti-CD3 antibody [UCHT1], prediluted (ab157300)
- Biotin Anti-CD3 antibody [UCHT1] (ab191112)
- APC Anti-CD3 antibody [UCHT1] (ab239287)
- redFluor™ 710 Anti-CD3 antibody [UCHT1] (ab241935)
- violetFluor™ 450 Anti-CD3 antibody [UCHT1] (ab241941)
- FITC Anti-CD3 antibody [UCHT1] (ab34275)
- PE/Cy7® Anti-CD3 antibody [UCHT1] (ab81992)
- APC/Cy7® Anti-CD3 antibody [UCHT1] (ab82013)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab157317 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use 4µl for 106 cells.
(or 100 µl of whole blood). ab157226 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
Flow Cyt (Intra) |
Use 4µl for 106 cells.
(or 100 µl of whole blood). ab157226 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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Flow Cyt
Use 4µl for 106 cells. (or 100 µl of whole blood). ab157226 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Flow Cyt (Intra)
Use 4µl for 106 cells. (or 100 µl of whole blood). ab157226 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab157317 has been referenced in 4 publications.
- Siegers GM et al. Different composition of the human and the mouse gammadelta T cell receptor explains different phenotypes of CD3gamma and CD3delta immunodeficiencies. J Exp Med 204:2537-44 (2007). PubMed: 17923503
- Rieux-Laucat F et al. Inherited and somatic CD3zeta mutations in a patient with T-cell deficiency. N Engl J Med 354:1913-21 (2006). PubMed: 16672702
- Arnett KL et al. Crystal structure of a human CD3-epsilon/delta dimer in complex with a UCHT1 single-chain antibody fragment. Proc Natl Acad Sci U S A 101:16268-73 (2004). PubMed: 15534202
- le Gouvello S et al. Serine 16 of stathmin as a cytosolic target for Ca2+/calmodulin-dependent kinase II after CD2 triggering of human T lymphocytes. J Immunol 161:1113-22 (1998). PubMed: 9686569