Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895)
Overview
- Product nameAnti-VDAC1 / Porin antibody - Mitochondrial Loading ControlSee all VDAC1 / Porin primary antibodies ...
- DescriptionRabbit polyclonal to VDAC1 / Porin - Mitochondrial Loading Control
- SpecificityThis antibody detects all 3 isoforms of VDAC.
- Tested applicationsWB, IHC-P, ICC/IF more details
- Species reactivityReacts with: Mouse, Rat, Chicken, Dog, Human, Zebrafish, Chinese Hamster
Predicted to work with: Rabbit, Cow, Pig
- Immunogen
Synthetic peptide derived from residues 150 - 250 of Human VDAC1 / Porin. The immunogen sequence is completely conserved between isoforms 1, 2 and 3 of human VDAC (Note: the amino acid sequence is proprietary; Peptide available as ab16131.)
- Positive controlThis antibody gave a positive control in the following human whole cell lysates: HeLa A431 Jurkat HEK293 This antibody gave a positive control in HeLa (Human epithelial carcinoma cell line) Nuclear This antibody gave a positive control in the following mouse tissue lysates: Heart Kidney Skeletal Muscle Spinal Cord This antibody gave a positive control in the following rat lysates: PC12 Whole Cell Brain Tissue Lysate - normal tissue Kidney Whole Cell Lysate - normal tissue
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab15895 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).Can be blocked with VDAC1 / Porin peptide (ab16131). |
| IHC-P | IHC-P: Use at an assay dependent dilution. |
| ICC/IF | ICC/IF: Use a concentration of 5 µg/ml. |
Target
- FunctionForms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.
- Tissue specificityHeart, liver and skeletal muscle.
- Sequence similaritiesBelongs to the eukaryotic mitochondrial porin family.
- DomainConsists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands. The helical N-terminus folds back into the pore opening and plays a role in voltage-gated channel activity.
- Cellular localizationMitochondrion outer membrane. Cell membrane.
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Database links
- Entrez Gene: 416320 Chicken
- Entrez Gene: 282119 Cow
- Entrez Gene: 7416 Human
- Entrez Gene: 22333 Mouse
- Entrez Gene: 397010 Pig
- Entrez Gene: 83529 Rat
- Entrez Gene: 334582 Zebrafish
- Omim: 604492 Human
- SwissProt: P45879 Cow
- SwissProt: P21796 Human
- SwissProt: Q60932 Mouse
- SwissProt: Q9MZ16 Pig
- SwissProt: Q9TT15 Rabbit
- SwissProt: Q9Z2L0 Rat
- SwissProt: Q6NWC1 Zebrafish
- Unigene: 519320 Human
- Unigene: 709372 Human
- Unigene: 3555 Mouse
- Unigene: 470023 Mouse
- Unigene: 54594 Rat
see all
Target information above from: UniProt accession
P21796
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
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Alternative names
- hVDAC1 antibodyMGC111064 antibodyMitochondrial Porin antibody
- Outer mitochondrial membrane protein porin 1 antibodyPlasmalemmal porin antibodyPorin 31HL antibodyPorin 31HM antibodyPorin antibodyPORIN-31-HL antibodyVDAC 1 antibodyVDAC antibodyVDAC-1 antibodyVdac1 antibodyVDAC1_HUMAN antibodyVoltage Dependent Anion Channel 1 antibodyVoltage dependent anion selective channel protein 1 antibodyVoltage-dependent anion-selective channel protein 1 antibody
see all
Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control images
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Western blot - VDAC1 / Porin antibody (ab15895)All lanes : Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895) at 1 µg/ml
Lane 1 : Hela Nuclear lysate
Lane 2 : Hela cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Jurkat cell lysate
Lane 5 : HEK293 cell lysate
Lane 6 : Hela Nuclear lysate withVDAC1 / Porin peptide (ab16131) at 1 µg/ml
Lane 7 : Hela cell lysate withVDAC1 / Porin peptide (ab16131) at 1 µg/ml
Lane 8 : A431 cell lysate withVDAC1 / Porin peptide (ab16131) at 1 µg/ml
Lane 9 : Jurkat cell lysate withVDAC1 / Porin peptide (ab16131) at 1 µg/ml
Lane 10 : HEK293 cell lysate withVDAC1 / Porin peptide (ab16131) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 31 kDa
Observed band size : 31 kDa -
Immunocytochemistry/ Immunofluorescence - VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895)ICC/IF image of ab15895 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab15895, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 phalloidin was used to label F-actin (red).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895)Image courtesy of Human Protein AtlasParaffin embedded sections of human kidney were incubated with ab15895 (1/600 dilution) at room temperature for 30 mins. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab15895 was also tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further images can be found at www.proteinatlas.org
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Western blot - VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895)All lanes : Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895) at 1 µg/ml
Lane 1 : Heart (Mouse) Tissue Lysate
Lane 2 : Kidney (Mouse) Tissue Lysate
Lane 3 :Skeletal Muscle (Mouse) Tissue Lysate (ab29711)
Lane 4 : Spinal Cord (Mouse) Tissue Lysate
Lane 5 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 6 : Brain (Rat) Tissue Lysate - normal tissue
Lane 7 :Kidney (Rat) Whole Cell Lysate - normal tissue (ab29480)
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 31 kDa
Observed band size : 31 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895)This image is courtesy of an anonymous Abreviewab15895 staining mouse heart tissue section by IHC-P. Sections were PFA fixed and subjected to heat mediated antigen retrieval in citrate buffer (pH 6) prior to blocking in 10% serum for 10 minutes at 25°C. The primary antibody was diluted 1/600 and incubated with the sample for 1 hour at 25°C. A biotinylated goat anti-rabbit antibody diluted 1/400 was used as the secondary.
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Western blot - VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895)All lanes : Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895) at 1 µg/ml
Lane 1 : Adult wild type zebrafish brain mitochondrial lysate at 20 µg
Lane 2 : Adult wild type zebrafish brain cytosolic lysate at 30 µg
Secondary
HRP-conjugated goat F(ab’)2 Fragment at 1/10000 dilution
developed using the ECL technique
Predicted band size : 31 kDa
Observed band size : 28,29 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 minuteImage courtesy of an anonymous Abreview.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895)Image courtesy of Dr Markus Kipp by Abreview.ab15895 staining VDAC1 / Porin in human cerebellum tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Tissue was fixed in paraformaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer pH 6.0. Samples were then incubated with ab15895 at a 1/1000 dilution for 12 hours at 4°C. The secondary used was an undiluted HRP conjugated anti-rabbit polyclonal. Note: The big cells are purkinje cells of the cerebellum, (no nuclear staining). -
Western blot - Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895)
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 31 kDa
Exposure time : 3 minutes -
Western blotAll lanes : Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895) at 1 µg/ml
Lane 1 : Chicken liver cell lysate
Lane 2 : CHO K1 cell lysate
Lane 3 : MDCK cell lysate
Lane 4 : Chicken liver cell lysate withVDAC1 / Porin peptide (ab16131) at 1 µg/ml
Lane 5 : CHO K1 cell lysate withVDAC1 / Porin peptide (ab16131) at 1 µg/ml
Lane 6 : MDCK cell lysate withVDAC1 / Porin peptide (ab16131) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
Alexa fluor goat polyclonal anti-Rabbit IgG at 1/10000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 31 kDa
Observed band size : 31 kDa
References for Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895)
This product has been referenced in:
- Brennan GP et al. Transgenic overexpression of 14-3-3 zeta protects hippocampus against endoplasmic reticulum stress and status epilepticus in vivo. PLoS One 8:e54491 (2013). WB ; Mouse . Read more (PubMed: 23359526) »
- Lapierre LA et al. Phosphorylation of Rab11-FIP2 regulates polarity in MDCK cells. Mol Biol Cell 23:2302-2318 (2012). Dog . Read more (PubMed: 22553350) »
