Overview
- Product nameAnti-HDAC3 antibodySee all HDAC3 primary antibodies ...
- DescriptionRabbit polyclonal to HDAC3
- Tested applicationsIHC-P, ICC/IF, ICC, WB, IP more details
- Species reactivityReacts with: Mouse, Rat, Human, Indian Muntjac, Zebrafish
Predicted to work with: Chicken, Xenopus laevis - Immunogen
Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human HDAC3.
(Peptide available as ab16279.)
- Positive controlThis antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; A431; HEK293 and Hela Nuclear lysate.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab16047 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| IHC-P | IHC-P: Use at an assay dependent concentration. |
| ICC/IF | ICC/IF: Use at an assay dependent concentration. |
| ICC | ICC: 1/100 - 1/500. |
| WB | WB: Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).Can be blocked with HDAC3 peptide (ab16279). |
| IP | IP: Use at an assay dependent concentration. |
Target
- FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation.
- Tissue specificityWidely expressed.
- Sequence similaritiesBelongs to the histone deacetylase family. HD type 1 subfamily.
- Post-translational
modificationsSumoylated in vitro. - Cellular localizationNucleus.
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Database links
- Entrez Gene: 8841 Human
- Entrez Gene: 15183 Mouse
- Entrez Gene: 84578 Rat
- Entrez Gene: 393965 Zebrafish
- Omim: 605166 Human
- SwissProt: O15379 Human
- SwissProt: O88895 Mouse
- SwissProt: Q6P6W3 Rat
- SwissProt: Q6IRL9 Xenopus laevis
- SwissProt: Q803C3 Zebrafish
- Unigene: 519632 Human
- Unigene: 20521 Mouse
- Unigene: 17284 Rat
- Unigene: 65911 Xenopus laevis
- Unigene: 80607 Zebrafish
see all
Target information above from: UniProt accession
O15379
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
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Alternative names
- HD 3 antibodyHD3 antibodyHDAC 3 antibody
- HDAC3 antibodyHDAC3_HUMAN antibodyHistone deacetylase 3 (HD3) (RPD3-2) antibodyhistone deacetylase 3 antibodyRPD 3 antibodyRPD3 2 antibodyRPD3 antibodyRPD3-2 antibodySMAP 45 antibodySMAP45 antibody
see all
Anti-HDAC3 antibody images
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Western blot - HDAC3 antibody (ab16047)All lanes : Anti-HDAC3 antibody (ab16047) at 1 µg/ml
Lane 1 : Mouse 3T3 cell lysate
Lane 2 : Rat liver cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 50 kDa
Observed band size : 49 kDa (why is the actual band size different from the predicted?)
Exposure time : 30 seconds -
Western blotAll lanes : Anti-HDAC3 antibody (ab16047) at 1 µg/ml
Lane 1 : HeLa nuclear lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Jurkat cell lysate
Lane 5 : HEK293 cell lysate
Lane 6 : HeLa nuclear lysate withHDAC3 peptide (ab16279) at 1 µg/ml
Lane 7 : HeLa whole cell lysate withHDAC3 peptide (ab16279) at 1 µg/ml
Lane 8 : A431 cell lysate withHDAC3 peptide (ab16279) at 1 µg/ml
Lane 9 : Jurkat cell lysate withHDAC3 peptide (ab16279) at 1 µg/ml
Lane 10 : HEK293 cell lysate withHDAC3 peptide (ab16279) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size : 50 kDa
Observed band size : 49 kDa (why is the actual band size different from the predicted?)
Exposure time : 30 seconds -
Immunoprecipitation - Anti-HDAC3 antibody (ab16047)HDAC3 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to HDAC3 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab16047.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 49kDa: HDAC3. -
Immunocytochemistry - HDAC3 antibody (ab16047)Darin McDonald, Cross Cancer InstituteSK-N-SH cells were fixed in 4% paraformaldehyde, permeabilized in 0.5% Triton X-100 and incubated for 1 hour with ab16047 (1/100). The antibody clearly stained the nucleus (red). The cells were counterstained with DAPI (blue). 100x magnification.
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Immunocytochemistry - HDAC3 antibody (ab16047)Kirk McManus, University of British ColumbiaInterphase HeLa cells incubated with ab16047 (1/500). The antibody shows predominantly nuclear staining. The ab16047 staining is shown in green. The cells were counterstained with DAPI (red).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HDAC3 antibody (ab16047)This image is courtesy of an Abreview submitted by Antibody Solutions Ltd.ab16047 staining HDAC3 in human breast cancer tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in Citrate buffer pH 6.0 and blocking (5 minutes/peroxidase block then 10 minutes/protein block) for 15 minutes at 20°C. The primary antibody was diluted, 1/2000 and incubated with sample for 45 minutes at 20°C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary.
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Western blot - Anti-HDAC3 antibody (ab16047)All lanes : Anti-HDAC3 antibody (ab16047) at 1 µg/ml
Lane 1 : Marker
Lane 2 : Zebrafish brain homogenate (20ug)
Lane 3 : Zebrafish heart homogenate (20ug)
Lane 4 : Zebrafish liver homogenate (20ug)
Secondary
Donkey polyclonal to Goat IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 50 kDa
Observed band size : 48 kDa (why is the actual band size different from the predicted?)
Exposure time : 5 minutes
References for Anti-HDAC3 antibody (ab16047)
This product has been referenced in:
- Samant SA et al. HDAC3-dependent Reversible Lysine Acetylation of Cardiac Myosin Heavy Chain Isoforms Modulates Their Enzymatic and Motor Activity. J Biol Chem 286:5567-77 (2011). Read more (PubMed: 21177250) »
- Brunmeir R et al. Epigenetic regulation of a murine retrotransposon by a dual histone modification mark. PLoS Genet 6:e1000927 (2010). WB ; Mouse . Read more (PubMed: 20442873) »
