Anti-Amino-terminal enhancer of split antibody (ab16638)

Overview

• Product nameAnti-Amino-terminal enhancer of split antibodySee all Amino-terminal enhancer of split primary antibodies ...
• Description
  Rabbit polyclonal to Amino-terminal enhancer of split
• Tested applicationsIP, WB more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human GRG (Groucho homologue).

  Read Abcam's proprietary immunogen policy

  (Peptide available as ab16684.)

• Positive controlThis antibody gives a positive signal in WB against Recombinant His-tagged GRG

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Stem Cells
  • Signaling Pathways
  • Notch
  • Nuclear

  • Stem Cells
  • Signaling Pathways
  • Wnt
  • Nuclear

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Co-factors

Applications

Target

• FunctionActs as dominant repressor towards other family members. Inhibits NF-kappa-B-regulated gene expression. May be required for the initiation and maintenance of the differentiated state.
• Tissue specificityFound predominantly in muscle, heart and Placenta. In fetal tissues, abundantly expressed in the heart, lung, kidney, brain and liver.
• Sequence similaritiesBelongs to the WD repeat Groucho/TLE family.
• DomainLacks the C-terminal WD repeats.
• Post-translational
  modificationsUbiquitinated by XIAP/BIRC4.
• Cellular localizationNucleus.

Target information above from: UniProt accession Q08117 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 166 Human
  • Entrez Gene: 14797 Mouse
  • Entrez Gene: 29466 Rat
  • Omim: 600188 Human
  • SwissProt: Q08117 Human
  • SwissProt: P63002 Mouse
  • SwissProt: P63003 Rat
  • Unigene: 515053 Human

  • Unigene: 180013 Mouse
  • Unigene: 11495 Rat

  see all

• Alternative names
  Aes 1 antibodyAes 2 antibodyaes antibody

  AES_HUMAN antibodyAes1 antibodyAes2 antibodyAmino Enhancer of Split antibodyAmino terminal enhancer of split antibodyAmino-terminal enhancer of split antibodyEsp 1 antibodyEsp1 antibodyGp130 associated protein GAM antibodyGp130-associated protein GAM antibodyGRG 5 antibodyGRG antibodyGRG protein antibodyGRG5 antibodyProtein ESP 1 antibodyProtein ESP1 antibodyProtein GRG antibodyTLE 5 antibodyTLE5 antibody

  see all

Anti-Amino-terminal enhancer of split antibody images

• 

  Western blot - GRG (Groucho homologue) antibody (ab16638)
  All lanes : Anti-Amino-terminal enhancer of split antibody (ab16638) at 1 µg/ml
  
  Lane 1 : As above
  Lane 2 : As above
  Lane 3 : As above
  Lane 4 : As above
  Lane 5 : As above
  Lane 6 : GRG (Groucho homologue) peptide (ab16684) at 1 µg/ml
  Lane 7 : GRG (Groucho homologue) peptide (ab16684) at 1 µg/ml
  Lane 8 : GRG (Groucho homologue) peptide (ab16684) at 1 µg/ml
  Lane 9 : GRG (Groucho homologue) peptide (ab16684) at 1 µg/ml
  Lane 10 : GRG (Groucho homologue) peptide (ab16684) at 1 µg/ml
  
  
  Performed under reducing conditions.
  
  Predicted band size : 22 kDa
  Observed band size : 21 kDa (why is the actual band size different from the predicted?)
  Additional bands at : 26 kDa (possible cross reactivity),28 kDa (possible cross reactivity),33 kDa (possible cross reactivity),40 kDa (possible cross reactivity),53 kDa (possible cross reactivity),75 kDa (possible cross reactivity),80 kDa (possible cross reactivity).