Anti-DOK2 antibody (ab1677)

Overview

• Product nameAnti-DOK2 antibodySee all DOK2 primary antibodies ...
• Description
  Rabbit polyclonal to DOK2
• SpecificityBinds to a 55-60 kDa protein.
• Tested applicationsIHC-P, WB, ELISA, ICC more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse
• Immunogen

  20 amino acid C-terminal peptide to p56 dok2 RasGAP-binding protein

• Positive controlHuman U937 cells or T-cell line Jurkatt.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPBS, 1mg/ml BSA, 0.1% sodium azide, pH7.2
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Signal transduction
  • Protein phosphorylation
  • Tyrosine kinases
  • Other

  • Cancer
  • Signal transduction
  • Other

  • Signal Transduction
  • Adapters
  • Cytoplasmic

  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • MAPK Pathway

Applications

Target

• FunctionDOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation.
• Tissue specificityHighly expressed in peripheral blood leukocytes, lymph nodes and spleen. Lower expression in thymus, bone marrow and fetal liver.
• Sequence similaritiesBelongs to the DOK family. Type A subfamily.
  Contains 1 IRS-type PTB domain.
  Contains 1 PH domain.
• DomainPTB domain mediates receptor interaction.
• Post-translational
  modificationsOn immunoreceptor stimulation, phosphorylated on C-terminal tyrosine residues. Phosphorylation on Tyr-345 is required for binding to the SH2 domain of NCK. Phosphorylation on both Tyr-271 and Tyr-299 is required for interaction with RASGAP.

Target information above from: UniProt accession O60496 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 9046 Human
  • Entrez Gene: 13449 Mouse
  • Omim: 604997 Human
  • SwissProt: O60496 Human
  • SwissProt: O70469 Mouse
  • Unigene: 71215 Human
  • Unigene: 243323 Mouse

• Alternative names
  Docking protein 2 56kDa antibodyDocking protein 2 antibodyDOK 2 antibody

  DOK R antibodyDOK R antibodyDOK2 antibodyDOK2_HUMAN antibodyDownstream of tyrosine kinase 2 antibodyOTTHUMP00000161710 antibodyOTTHUMP00000224923 antibodyp56(dok 2) antibodyp56(dok-2) antibodyp56dok 2 antibodyp56DOK antibodyp56dok2 antibody

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Anti-DOK2 antibody images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-DOK2 antibody(ab1677)
  Ab1677 staining human breast carcinoma. Staining is localized to nucleus and cytoplasm.
  Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
  Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.