Anti-TAG72 antibody [CC49] (ab16838)

Overview

• Product nameAnti-TAG72 antibody [CC49]See all TAG72 primary antibodies ...
• Description
  Mouse monoclonal [CC49] to TAG72
• SpecificityThis antibody has been shown to be distinct from B72.3 (ab691, ab1320-prediluted) by reciprocal competition radio-immunoassays and it has a Ka higher than that of B72.3.
• Tested applicationsIHC-P, ICC/IF more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Full length native protein (purified).

• Positive controlBreast carcinoma

Properties

• FormLiquid
• Storage instructionsStore at +4°C.
• Storage bufferPreservative: 0.05% Sodium Azide
  Constituents: 1% BSA, Tissue culture supernatant
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberCC49
• IsotypeIgG1
• Research Areas
  • Cancer
  • Tumor biomarkers
  • Tumor antigens

  • Signal Transduction
  • Cytoskeleton / ECM
  • Extracellular Matrix
  • ECM Proteins
  • Tumor Glycoproteins

  • Tags & Cell Markers
  • Cell Type Markers
  • Tumor Associated

Applications

Target

• RelevanceThe majority of human adenocarcinomas including colorectal, pancreatic, gastric, ovarian, endometrial, mammary, and non-small cell lung cancer display some cell populations that are positive for TAG72. About 60% of carcinoma patients express TAG72 in their sera. TAG72 is reportedly useful in distinguishing pulmonary adenocarcinomas from pleural mesotheliomas.
• Cellular localizationCytoplasmic

• Alternative names
  CA 72.4 antibodyTAG 72 antibodyTumor associated Glycoprotein 72 antibody

Anti-TAG72 antibody [CC49] images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TAG72 antibody [CC49] (ab16838)
  ab16838 staining human colon carcinoma by IHC-P.
• 

  Immunocytochemistry/ Immunofluorescence - Anti-TAG72 antibody [CC49] (ab16838)
  ICC/IF image of ab16838 stained MCF-7 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16838, undiluted) overnight at +4°C. The secondary antibody (green) was ab96879 Dylight 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.