Anti-Beclin 1 antibody (ab16998)

Overview

• Product nameAnti-Beclin 1 antibodySee all Beclin 1 primary antibodies ...
• Description
  Rabbit polyclonal to Beclin 1
• Tested applicationsICC/IF, WB, IP, IHC-P more details
• Species reactivity
  Reacts with: Mouse, Human
  Predicted to work with: Rat
• Immunogen

  Synthetic peptide (Human) (Internal sequence within residues 1-100).

Properties

• FormLiquid
• Storage instructionsStore at +4°C.
• Storage bufferPreservative: 0.1% Sodium Azide
  Constituents: Tris buffered saline, 5mg/ml BSA
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Metabolism processes
  • Autophagy and mitophagy

  • Cancer
  • Oncoproteins/suppressors
  • Tumor suppressors
  • Other

  • Cancer
  • Signal transduction
  • Autophagy

  • Microbiology
  • Interspecies Interaction
  • Host Immune Response

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Cancer susceptibility
  • Tumor Suppressors

Applications

Target

• FunctionPlays a central role in autophagy (By similarity). May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.
• Tissue specificityUbiquitous.
• Sequence similaritiesBelongs to the beclin family.
• Cellular localizationGolgi apparatus > trans-Golgi network membrane. Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells.

Target information above from: UniProt accession Q14457 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 8678 Human
  • Entrez Gene: 56208 Mouse
  • Entrez Gene: 114558 Rat
  • Omim: 604378 Human
  • SwissProt: Q14457 Human
  • SwissProt: O88597 Mouse
  • SwissProt: Q91XJ1 Rat
  • Unigene: 716464 Human

  • Unigene: 178947 Mouse
  • Unigene: 2776 Rat

  see all

• Alternative names
  APG6 antibodyATG 6 antibodyATG6 antibody

  ATG6 autophagy related 6 homolog antibodyBcl-2-interacting protein beclin antibodyBeclin 1 (coiled coil moesin like BCL2 interacting protein) antibodyBeclin 1 autophagy related antibodyBeclin-1 antibodyBeclin1 antibodyBECN 1 antibodyBECN1 antibodyBECN1_HUMAN antibodyCoiled coil myosin like BCL2 interacting protein antibodyCoiled-coil myosin-like BCL2-interacting protein antibodyGT197 antibodyProtein GT197 antibodyVPS 30 antibodyVPS30 antibody

  see all

Anti-Beclin 1 antibody images

• 

  Western blot - Beclin 1 antibody (ab16998)
  Anti-Beclin 1 antibody (ab16998) + Mouse liver tissue lysate at 50 µg
  
  Predicted band size : 52 kDa
  Observed band size : 52 kDa
  
• 

  Immunocytochemistry/ Immunofluorescence-Beclin 1 antibody(ab16998)
  ICC/IF image of ab16998 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16998, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.