Anti-pan Cytokeratin antibody [KL1] (ab17154)

Overview

• Product nameAnti-pan Cytokeratin antibody [KL1]See all pan Cytokeratin primary antibodies ...
• Description
  Mouse monoclonal [KL1] to pan Cytokeratin
• SpecificityReacts with polypeptides of 65.5, 58, 56, 52.5, 52, 45, 40kD. Immunoblotting analysis revealed that this antibody reacts with cytokeratin 1, 2, 5, 6, 7, 8, 11, 14, 16, 17 and 18. This antibody stains with neoplastic cells of epithelial origin. Non-epithelial cells and skin basal layers cells do not stain with this antibody.
• Tested applicationsIHC-P more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Human keratin isolated from epidermal stratum corneum.

• Positive controlSkin

Properties

• FormLiquid
• Storage instructionsStore at +4°C.
• Storage bufferPreservative: 0.05% Sodium Azide
  Constituents: 1% BSA
• Concentration information loading...
• PurityProtein G purified
• Clonality Monoclonal
• Clone numberKL1
• IsotypeIgG1
• Light chain typekappa
• Research Areas
  • Signal Transduction
  • Cytoskeleton / ECM
  • Cytoskeleton
  • Intermediate Filaments
  • Class I
  • Cytokeratins

Applications

Target

• RelevanceCytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.
• Cellular localizationCytoplasmic

Anti-pan Cytokeratin antibody [KL1] images

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  Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - pan Cytokeratin antibody [KL1] (ab17154)
  Formalin fixed paraffin embedded human lung carcinoma stained with pan Cytokeratin using ABC and AEC chromogen.