Anti-XAF1 antibody (ab17204)

Overview

• Product nameAnti-XAF1 antibodySee all XAF1 primary antibodies ...
• Description
  Rabbit polyclonal to XAF1
• Tested applicationsICC/IF, WB, IHC-P more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Synthetic peptide (Human) (within the last 35 amino acids of C terminal end).

• Positive controlHuman spleen cell lysate.

Properties

• FormLiquid
• Storage instructionsStore at +4°C.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: PBS
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cell Biology
  • Apoptosis
  • Intracellular
  • Caspases etc
  • Inhibitors

Applications

Target

• FunctionSeems to function as a negative regulator of members of the IAP (inhibitor of apoptosis protein) family. Inhibits anti-caspase activity of BIRC4. Induces cleavage and inactivation of BIRC4 independent of caspase activation. Mediates TNF-alpha-induced apoptosis and is involved in apoptosis in trophoblast cells. May inhibit BIRC4 indirectly by activating the mitochondrial apoptosis pathway. After translocation to mitochondra, promotes translocation of BAX to mitochondria and cytochrome c release from mitochondria. Seems to promote the redistribution of BIRC4 from the cytoplasm to the nucleus, probably independent of BIRC4 inactivation which seems to occur in the cytoplasm. The BIRC4-XAF1 complex mediates down-regulation of BIRC5/survivin; the process requires the E3 ligase activity of BIRC4. Seems to be involved in cellular sensitivity to the proapoptotic actions of TRAIL. May be a tumor suppressor by mediating apoptosis resistance of cancer cells.
• Tissue specificityWidely expressed. Expression is frequently down-regulated in cancer cell lines. Isoform 5 is widely expressed. Expressed in placenta (at protein level).
• Sequence similaritiesContains 1 TRAF-type zinc finger.
• Developmental stageSelectively expressed in third trimester placenta.
• Cellular localizationNucleus and Cytoplasm. Nucleus. Mitochondrion. Found in the cytoplasm and nucleus of placental syncytiotrophoblasts. Translocates to mitochondria upon TNF-alpha treatment.

Target information above from: UniProt accession Q6GPH4 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 54739 Human
  • Entrez Gene: 54739 Human
  • Entrez Gene: 327959 Mouse
  • Entrez Gene: 679600 Rat
  • Omim: 606717 Human
  • SwissProt: Q6GPH4 Human
  • SwissProt: Q6GPH4 Human
  • SwissProt: Q5NBU8 Mouse

  • Unigene: 441975 Human
  • Unigene: 291131 Mouse

  see all

• Alternative names
  BIRC 4 binding protein antibodyBIRC4 binding protein antibodyBIRC4-binding protein antibody

  BIRC4BP antibodyHSXIAPAF 1 antibodyHSXIAPAF1 antibodyXAF 1 antibodyXAF1 antibodyXAF1_HUMAN antibodyXIAP associated factor 1 antibodyXIAP-associated factor 1 antibodyXIAPAF1 antibody

  see all

Anti-XAF1 antibody images

• 

  Western blot - XAF1 antibody (ab17204)
  Lane 1 : Anti-XAF1 antibody (ab17204) at 0.5 µg/ml
  Lane 2 : Anti-XAF1 antibody (ab17204) at 1 µg/ml
  Lane 3 : Anti-XAF1 antibody (ab17204) at 2 µg/ml
  
  Lane 1 : Human spleen lysate
  Lane 2 : Human spleen lysate
  Lane 3 : Human spleen lysate
  
  
  Observed band size : 32 kDa (why is the actual band size different from the predicted?)
  
  
  Higher 50kDa band presence is lot dependent. This band is not blocked by the immunogen.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - XAF1 antibody (ab17204)

  ab17204 at 2µg/ml staining XAF1 in human spleen tissue by IHC

• 

  Immunocytochemistry/ Immunofluorescence-XAF1 antibody(ab17204)
  ICC/IF image of ab17204 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab17204, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.