Anti-CIAS1 / NALP3 antibody [nalpy3-b] (ab17267)

Overview

• Product nameAnti-CIAS1 / NALP3 antibody [nalpy3-b]See all CIAS1 / NALP3 primary antibodies ...
• Description
  Mouse monoclonal [nalpy3-b] to CIAS1 / NALP3
• SpecificityRecognizes human NALP3. Detects endogenous protein by IP and WB.
• Tested applicationsIHC-P, IHC-Fr, IP, WB more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Recombinant human NALP3 (NACHT-, LRR- and PYD-containing protein 3) (pyrin domain).

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: PBS
• Concentration information loading...
• Purity>95% by SDS-PAGE
• Clonality Monoclonal
• Clone numbernalpy3-b
• IsotypeIgG1
• Light chain typeunknown
• Research Areas
  • Cancer
  • Growth factors
  • TNF

  • Signal Transduction
  • Signaling Pathway
  • Nuclear Signaling
  • NFkB Pathway

  • Cardiovascular
  • Atherosclerosis
  • Vascular Inflammation
  • Inflammatory mediators

  • Immunology
  • Innate Immunity
  • Macrophage / Inflamm.

Applications

Target

• FunctionMay function as an inducer of apoptosis. Interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling. Inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. Activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18.
• Tissue specificityExpressed in blood leukocytes. Strongly expressed in polymorphonuclear cells and osteoblasts. Undetectable or expressed at a lower magnitude in B- and T-lymphoblasts, respectively. High level of expression detected in chondrocytes. Detected in non-keratinizing epithelia of oropharynx, esophagus and ectocervix and in the urothelial layer of the bladder.
• Involvement in diseaseDefects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1) [MIM:120100]; also known as familial cold urticaria. FCAS are rare autosomal dominant systemic inflammatory diseases characterized by episodes of rash, arthralgia, fever and conjunctivitis after generalized exposure to cold.
  Defects in NLRP3 are a cause of Muckle-Wells syndrome (MWS) [MIM:191900]; also known as urticaria-deafness-amyloidosis syndrome. MWS is a hereditary periodic fever syndrome characterized by fever, chronic recurrent urticaria, arthralgias, progressive sensorineural deafness, and reactive renal amyloidosis. The disease may be severe if generalized amyloidosis occurs.
  Defects in NLRP3 are the cause of chronic infantile neurologic cutaneous and articular syndrome (CINCA) [MIM:607115]; also known as neonatal onset multisystem inflammatory disease (NOMID). CINCA is a rare congenital inflammatory disorder characterized by a triad of neonatal onset of cutaneous symptoms, chronic meningitis and joint manifestations with recurrent fever and inflammation.
• Sequence similaritiesBelongs to the NLRP family.
  Contains 1 DAPIN domain.
  Contains 9 LRR (leucine-rich) repeats.
  Contains 1 NACHT domain.
• Cellular localizationCytoplasm.

Target information above from: UniProt accession Q96P20 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 114548 Human
  • Entrez Gene: 114548 Human
  • Entrez Gene: 114548 Human
  • Omim: 606416 Human
  • SwissProt: Q96P20 Human
  • SwissProt: Q96P20 Human
  • SwissProt: Q96P20 Human
  • Unigene: 159483 Human

• Alternative names
  AGTAVPRL antibodyAII/AVP antibodyAngiotensin/vasopressin receptor AII/AVP like antibody

  Angiotensin/vasopressin receptor AII/AVP-like antibodyC1orf7 antibodyCaterpiller protein 1.1 antibodyCIAS 1 antibodyCIAS1 antibodyCLR1.1 antibodyCold autoinflammatory syndrome 1 antibodyCold autoinflammatory syndrome 1 protein antibodyCryopyrin antibodyFamilial cold autoinflammatory syndrome antibodyFCAS antibodyFCU antibodyLRR and PYD domains-containing protein 3 antibodyMuckle-Wells syndrome antibodyMWS antibodyNACHT antibodyNACHT LRR and PYD containing protein 3 antibodyNALP 3 antibodyNALP3 antibodyNALP3_HUMAN antibodyNLRP3 antibodyPYPAF 1 antibodyPYPAF1 antibodyPYRIN containing APAF1 like protein 1 antibodyPYRIN-containing APAF1-like protein 1 antibody

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Anti-CIAS1 / NALP3 antibody [nalpy3-b] images

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  Immunohistochemistry (Frozen sections) - CIAS1 / NALP3 antibody [nalpy3-b] (ab17267)
  Staining of endogenous NALP3 in epithelial layer of human tonsil (frozen section) using ab17267. Method: 5μM frozen sections of tissue are dried and fixed with acetone. Tissue is washed with PBS and incubated with ab17267.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CIAS1 / NALP3 antibody [nalpy3-b] (ab17267)Image from Nour AM et al., J Biol Chem. 2011 May 20;286(20):17921-33. Epub 2011 Mar 8. Fig 5.; doi: 10.1074/jbc.M110.210575; May 20, 2011, The Journal of Biological Chemistry, 286, 17921-17933.
  Immunohistochemical analysis of Varicella-Zoster Virus-infected Human skin xenografts in SCID mice, staining CIAS1 / NALP3 (green) with ab17267.
  
  Antigen retrieval was performed using the pressure cooker method in the presence of sodium citrate, pH 6. Sections were blocked with 0.5% fish gelatin before staining with primary antibody. An AlexaFluor®488-conjugated anti-mouse IgG was used as the secondary antibody.