Overview
- Product nameAnti-HLTF antibody - ChIP GradeSee all HLTF primary antibodies ...
- DescriptionRabbit polyclonal to HLTF - ChIP Grade
- Tested applicationsChIP, WB, IP more details
- Species reactivityReacts with: Human
Predicted to work with: Mouse - Immunogen
Synthetic peptide.
- EpitopeThe epitope recognized by antibody maps to a region between residues 600 and 700 of human SMARC A3 using the number given in entry NP_003062.2 (GeneID 6596)
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.1% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8 -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesAntibody was affinity purified using an epitope specific to SMARCA3 immobilized on solid support.
- Clonality Polyclonal
- IsotypeIgG
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Research Areas
Applications
Our Abpromise guarantee covers the use of ab17984 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ChIP | ChIP: Use at an assay dependent dilution. |
| WB | WB: 1/3000 - 1/10000. Detects a band of approximately 125 kDa (predicted molecular weight: 114 kDa). |
| IP | IP: Use a concentration of 2 - 5 µg/ml. |
Target
- FunctionHas both helicasee and E3 ubiquitin ligase activities. Possesses intrinsic ATP-dependent nucleosome-remodeling activity; This activity may be required for transcriptional activation or repression of specific target promoters (By similarity). These may include the SERPINE1 and HIV-1 promoters and the SV40 enhancer, to which this protein can bind directly. Plays a role in error-free postreplication repair (PRR) of damaged DNA and maintains genomic stability through acting as a ubiquitin ligase for 'Lys-63'-linked polyubiquitination of chromatin-bound PCNA.
- Tissue specificityExpressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.
- PathwayProtein modification; protein ubiquitination.
- Sequence similaritiesBelongs to the SNF2/RAD54 helicase family. RAD16 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 RING-type zinc finger. - Cellular localizationNucleus.
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Database links
- Entrez Gene: 6596 Human
- Entrez Gene: 20585 Mouse
- Omim: 603257 Human
- SwissProt: Q14527 Human
- SwissProt: O35597 Mouse
- SwissProt: Q6PCN7 Mouse
- Unigene: 3068 Human
- Unigene: 209650 Mouse
Target information above from: UniProt accession
Q14527
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- DNA-binding protein/plasminogen activator inhibitor 1 regulator antibodyHelicase like transcription factor antibodyHelicase-like transcription factor antibody
- HIP116 antibodyHIP116A antibodyHLTF 1 antibodyHLTF antibodyHLTF_HUMAN antibodyHLTF1 antibodyp113 antibodyRING finger protein 80 antibodyRNF80 antibodySMARC A3 antibodySMARCA 3 antibodySMARCA3 antibodySNF2-like 3 antibodySNF2L3 antibodySucrose nonfermenting protein 2 like 3 antibodySucrose nonfermenting protein 2-like 3 antibodySWI/SNF related matrix associated actin dependent regulator of chromatin subfamily A member 3 antibodySWI/SNF-related matrix-associated actin-dependent regulator of chromatin a3 antibodySWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3 antibodyZBU 1 antibodyZBU1 antibody
see all
Anti-HLTF antibody - ChIP Grade images
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Western blot - SMARCA3 antibody - ChIP Grade (ab17984)
Predicted band size : 114 kDaDetection of SMARCA3 by western blot and immunoprecipitation.
Lane 1: 20µg of HeLa nuclear extract
Lane 2: 50µg of HeLa nuclear extract
LAne 3: 1 mg of HeLa nuclear extract immunoprecipitated by anti-SMARCA3 (ab17984), used at 4µg/mg of lysate.
Anti-SMARCA3 (ab17984) was used for western blot analysis at 1/5,000.
Detection: chemiluminescence with an exposure time of 4 minutes. -
ChIP - SMARCA3 antibody - ChIP Grade (ab17984)This image is courtesy of an Abreview submitted by Dr Beverly Chiltonab17984 in ChIP of rabbit endometrium nuclear cell lysate. In vivo binding of SMARCA3 (RUSH) to DNA and Egr-1/c-Rel in the rabbit RUSH promoter was confirmed by PCR amplification of products from ChIP assays.
Lane 1 FX174/HaeIII size markers.
Lane 2 Single 166-bp amplicons of the SMARCA3 (RUSH) site resulting from ChIP with antibodies to SMARCA3.
Lanes 3 Negative ChIP control (IgG)
Lane 4 PCR control (water)
Lane 5 Single 153-bp amplicons of the Sp1(A) site resulting from ChIP with antibodies to SMARCA3. The SMARCA3 (RUSH) site was identified through antibody-SMARCA3-DNA binding. The Sp1(A) site is an Egr-1/Sp1/3/MAZ/MZF1/c-Rel composite binding site that was identified through antibody-SMARCA3-Egr-1/c-Rel-DNA binding.
Cross-linking agent: disuccinimydyl glutarate and formaldehyde (0.75%)
References for Anti-HLTF antibody - ChIP Grade (ab17984)
This product has been referenced in:
- Helmer RA et al. Prolactin-induced Jak2 phosphorylation of RUSH: a key element in Jak/RUSH signaling. Mol Cell Endocrinol 325:143-9 (2010). Read more (PubMed: 20562009) »
- Hewetson A & Chilton BS Progesterone-Dependent Deoxyribonucleic Acid Looping between RUSH/SMARCA3 and Egr-1 Mediates Repression by c-Rel. Mol Endocrinol 22:813-22 (2008). Read more (PubMed: 18174357) »
