Anti-AP2 beta antibody [AP2b 1F3/8] (ab18113)

Overview

• Product nameAnti-AP2 beta antibody [AP2b 1F3/8]See all AP2 beta primary antibodies ...
• Description
  Mouse monoclonal [AP2b 1F3/8] to AP2 beta
• Tested applicationsWB, ELISA, IHC-Fr more details
• Species reactivity
  Reacts with: Rat, Human
  
• Immunogen

  Truncated AP-2 beta-protein prepared from bacteria.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: PBS
• Concentration information loading...
• PurityIgG fraction
• Clonality Monoclonal
• Clone numberAP2b 1F3/8
• IsotypeIgG2a
• Research Areas
  • Epigenetics and Nuclear Signaling
  • Transcription
  • Transcription Factors

  • Cell Biology
  • Cell Cycle
  • Cell differentiation

Applications

Target

• FunctionSequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-beta appears to be required for normal face and limb development and for proper terminal differentiation and function of renal tubular epithelia.
• Involvement in diseaseDefects in TFAP2B are the cause of Char syndrome (CHAR) [MIM:169100]. CHAR is an autosomal dominant disorder characterized by patent ductus arteriosus (PDA), facial dysmorphism and hand anomalies.
• Sequence similaritiesBelongs to the AP-2 family.
• Post-translational
  modificationsSumoylated on Lys-21; which inhibits transcriptional activity.
• Cellular localizationNucleus.

Target information above from: UniProt accession Q92481 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 7021 Human
  • Entrez Gene: 7021 Human
  • Entrez Gene: 7021 Human
  • Entrez Gene: 301285 Rat
  • Omim: 601601 Human
  • SwissProt: Q92481 Human
  • SwissProt: Q92481 Human
  • SwissProt: Q92481 Human

  • Unigene: 33102 Human

  see all

• Alternative names
  Activating enhancer binding protein 2 beta antibodyActivating enhancer-binding protein 2-beta antibodyAP 2B antibody

  AP2 B antibodyAP2-beta antibodyAP2B antibodyAP2B_HUMAN antibodyAP2beta antibodyMGC21381 antibodyOTTHUMP00000039925 antibodyTFAP 2B antibodyTFAP2B antibodyTranscription factor AP 2 beta antibodyTranscription factor AP-2-beta antibodyTranscription factor AP2 beta antibody

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Anti-AP2 beta antibody [AP2b 1F3/8] images

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  Immunohistochemistry (Frozen sections) - AP2 beta antibody [AP2b 1F3/8] (ab18113)This image is courtesy of an Abreview submitted by Dr Sophie Pezet

  ab18113 at 1/300 staining perfusion fixed rat brain tissue sections by IHC-Fr. Adult rat was perfused intracardially with paraformaldehyde 4% + 15% of a saturated solution of picric acid in PB 0.2M. The brain was post-fixed in the same fixative for 24 hours. Sections were cryoprotected with sucrose 20% and later frozen in OCT. Sections were incubated in free floating 12h with the primary antibody (1/300) and revealed with secondary antibody conjugated with Alexa Fluor ® 488 (1/2000). The staining obtained is restricted to the cytoplasm and consists of a small and thin punctate staining (white arrows). The pictures show the staining obtained in cortical neurons, with the X20 (top picture) and X40 (bottom picture) objective.

  See Abreview