Anti-ABCA1 antibody [AB.H10] (ab18180)

Overview

• Product nameAnti-ABCA1 antibody [AB.H10]See all ABCA1 primary antibodies ...
• Description
  Mouse monoclonal [AB.H10] to ABCA1
• Tested applicationsIHC-P, ICC/IF, Flow Cyt, WB, ELISA, IP more details
• Species reactivity
  Reacts with: Mouse, Rat, Chicken, Cow, Human
  
• Immunogen

  Recombinant fragment, corresponding to amino acids 1800-2260 of Human ABCA1.

• Positive controltestis, liver, and brain tissue (negative control: muscle tissue)

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.05% Sodium Azide
  Constituents: PBS, pH 7.4
• Concentration information loading...
• PurityIgG fraction
• Clonality Monoclonal
• Clone numberAB.H10
• IsotypeIgG1
• Research Areas
  • Metabolism
  • Types of disease
  • Heart disease

  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Lipid and lipoprotein metabolism
  • Lipid metabolism

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Lipid and lipoprotein metabolism
  • Cholesterol Metabolism

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Lipid and lipoprotein metabolism
  • Lipoprotein metabolism

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Metabolism of lipids and lipoproteins

  • Signal Transduction
  • Metabolism
  • Lipid metabolism

  • Cardiovascular
  • Atherosclerosis
  • Lipoprotein metabolism

  • Cardiovascular
  • Lipids / Lipoproteins
  • Lipid Metabolism
  • Cholesterol Metabolism

  • Neuroscience
  • Cell Type Marker
  • Glia marker
  • Astrocyte marker

Applications

Target

• FunctioncAMP-dependent and sulfonylurea-sensitive anion transporter. Key gatekeeper influencing intracellular cholesterol transport.
• Tissue specificityWidely expressed, but most abundant in macrophages.
• Involvement in diseaseDefects in ABCA1 are a cause of high density lipoprotein deficiency type 1 (HDLD1) [MIM:205400]; also known as analphalipoproteinemia or Tangier disease (TGD). HDLD1 is a recessive disorder characterized by absence of high density lipoprotein (HDL) cholesterol from plasma, accumulation of cholesteryl esters, premature coronary artery disease (CAD), hepatosplenomegaly, recurrent peripheral neuropathy and progressive muscle wasting and weakness.
  Defects in ABCA1 are a cause of high density lipoprotein deficiency type 2 (HDLD2) [MIM:604091]; also known as familial hypoalphalipoproteinemia (FHA). HDLD2 is inherited as autosomal dominant trait. It is characterized by moderately low HDL cholesterol, predilection toward premature coronary artery disease (CAD) and a reduction in cellular cholesterol efflux.
• Sequence similaritiesBelongs to the ABC transporter superfamily. ABCA family.
  Contains 2 ABC transporter domains.
• DomainMultifunctional polypeptide with two homologous halves, each containing an hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain.
• Post-translational
  modificationsPhosphorylation on Ser-2054 regulates phospholipid efflux.
  Palmitoylation by DHHC8 is essential for membrane localization.
• Cellular localizationMembrane.

Target information above from: UniProt accession O95477 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 373945 Chicken
  • Entrez Gene: 535379 Cow
  • Entrez Gene: 19 Human
  • Entrez Gene: 11303 Mouse
  • Entrez Gene: 313210 Rat
  • Omim: 600046 Human
  • SwissProt: O95477 Human
  • SwissProt: P41233 Mouse

  • Unigene: 429294 Human
  • Unigene: 277376 Mouse
  • Unigene: 148916 Rat

  see all

• Alternative names
  ABC 1 antibodyABC Transporter 1 antibodyABC-1 antibody

  ABC1 antibodyABCA 1 antibodyABCA1 antibodyABCA1_HUMAN antibodyATP binding Cassette 1 antibodyATP binding cassette sub family A ABC1 member 1 antibodyATP binding cassette sub family A member 1 antibodyATP binding cassette sub-family A member 1 antibodyATP binding Cassette Transporter 1 antibodyATP-binding Cassette 1 antibodyATP-binding cassette sub-family A member 1 antibodyATP-binding Cassette Transporter 1 antibodyCERP antibodyCholesterol Efflux Regulatory Protein antibodyFLJ14958 antibodyHDLDT1 antibodyMembrane bound antibodyMGC164864 antibodyMGC165011 antibodyTD antibodyTGD antibody

  see all

Anti-ABCA1 antibody [AB.H10] images

• 

  Western blot - Anti-ABCA1 antibody [AB.H10] (ab18180)
  
  
  Predicted band size : 254 kDa
  
  
  Western blot analysis of ABCA1 induction by cholesterol (+) in human fibroblast cells from different patients (1,2,3,4). Simultaneous blotting with anti-actin antibody was used for protein loading control.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ABCA1 antibody [AB.H10] (ab18180)
  Immunoreactivity of pancreas tissue for ABCA1 protein. Left panel: strong immunopositivity of exocrine glandular cells of the pancreas, especially in the basal region (arrows). Interstitial microvascular cells are apparently negative. Cells within the islet were weakly positive (data not shown). Right panel: the negative control. Hematoxylin counterstain.
• 

  Flow Cytometry - ABCA1 antibody [AB.H10] (ab18180)
  Overlay histogram showing HepG2 cells stained with ab18180 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18180, 2µg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.
  
  

Protocols

• Recommended protocol for IHC-P with ab18180