Anti-HA tag antibody [HA.C5] (ab18181)

Overview

• Product nameAnti-HA tag antibody [HA.C5]See all HA tag primary antibodies ...
• Description
  Mouse monoclonal [HA.C5] to HA tag
• SpecificityRecognizes HA-tagged proteins overexpressed in cells, including both amino- and carboxy-termini of targeted proteins in transfected mammalian cells.
• Tested applicationsChIP/Chip, WB, ICC, IP, ICC/IF more details
• Immunogen

  Synthetic peptide from influenza hemagglutinin epitope: YPYDVPDYA conjugated to KLH.

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Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.05% Sodium Azide
  Constituents: PBS, pH 7.4
• Concentration information loading...
• PurityIgG fraction
• Clonality Monoclonal
• Clone numberHA.C5
• IsotypeIgG3
• Research Areas
  • Tags & Cell Markers
  • Epitope Tags
  • HA Tag

Applications

Target

• RelevanceHuman influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

• Alternative names
  HA epitope tag antibodyhemagglutinin antibody

Anti-HA tag antibody [HA.C5] images

• 

  Western blot - HA tag antibody [HA.C5] (ab18181)
  Western blot using ab18181 of 293 cells transfected with HA-tagged vector(2) and untransfected control (1).
• 

  Immunocytochemistry/ Immunofluorescence - HA tag antibody [HA.C5] (ab18181)
  Immunofluorescence using ab18181 staining a HA-tag fusion protein (transcription factor) in a stable expressing cell line (right hand panel) and control cell line (left hand panel).
• 

  Western blot - HA tag antibody [HA.C5] (ab18181)
  All lanes : Anti-HA tag antibody [HA.C5] (ab18181) at 1/2000 dilution
  
  Lane 1 : WCE from cell line transfected for HA-tagged protein
  Lane 2 : WCE from a cell line transfected with empty vector
  
  Lysates/proteins at 50 µg per lane.
  
  Secondary
  HRP conjugated Goat anti-mouse IgG (H+L)
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Exposure time : 10 seconds

  This image is courtesy of an anonymous Abreview

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• 

  Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA.C5] (ab18181)This image is courtesy of an Abreview submitted by Harendra Chahar
  ab18181 staining HA tag (green) in HeLa cells by Immunocytochemistry/ Immunofluorescence.
  
  Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 3% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/1000 in diluent) for 1 hour at 22°C. A FITC-conjugated goat anti-mouse polyclonal IgG (1/1000) was used as the secondary antibody. Nuclei were stained with DAPI (blue).

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