Anti-6X His tag® antibody [HIS.H8] (ab18184)

Overview

• Product nameAnti-6X His tag® antibody [HIS.H8]See all 6X His tag® primary antibodies ...
• Description
  Mouse monoclonal [HIS.H8] to 6X His tag®
• SpecificityRecognizes His-tagged recombinant proteins or His-tagged proteins overexpressed in cells.
• Tested applicationsICC/IF, ELISA, Dot Blot, WB, IP, EMSA more details
• Immunogen

  6x His synthetic peptide.

• General notesHIS-TAG® is a trademark of EMD Biosciences, Inc.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.1% Sodium Azide
  Constituents: PBS, pH 7.2
• Concentration information loading...
• PurityIgG fraction
• Clonality Monoclonal
• Clone numberHIS.H8
• IsotypeIgG2b
• Research Areas
  • Tags & Cell Markers
  • Epitope Tags
  • His Tag

Applications

Target

• RelevanceThe H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
• Cellular localizationDepends upon the localization of the parent protein tagged with hexahistidine.

• Alternative names
  6 His epitope tag antibodyHexa His tag antibodyHHHHHH epitope tag antibody

  HHHHHH tag antibodyHis tag antibody

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Anti-6X His tag® antibody [HIS.H8] images

• 

  Immunocytochemistry/ Immunofluorescence - 6X His tag®, antibody [HIS.H8] (ab18184)

  Immunofluorescence (red) with ab18184 on His-tag fusion protein in 293 cells.
  Left hand panel: untransfected control
  Right hand panel: transfected.
  Counterstained with DAPI (blue).

• 

  Western blot - 6X His tag antibody [HIS.H8] (ab18184)
  All lanes : Anti-6X His tag® antibody [HIS.H8] (ab18184) at 1/1000 dilution
  
  Lane 1 : 293 cells, untransfected control
  Lane 2 : 293 cells transfected with His-tagged vector
  
• 

  Western blot - 6X His tag® antibody [HIS.H8] (ab18184)
  Anti-6X His tag® antibody [HIS.H8] (ab18184) at 1/5000 dilution + Conditioned medium from transfected HEK293 cells which produce recombinant secretory protein with a 6XHis-Tag at 5 µg
  
  Secondary
  An HRP-conjugated Horse anti mouse polyclonal at 1/5000 dilution

  This image is courtesy of an anonymous Abreview

  See Abreview

• 

  Immunocytochemistry/ Immunofluorescence - 6X His tag® antibody [HIS.H8] (ab18184)This image is courtesy of an anonymous abreview.
  ab18184 at a 1/1000 dilution staining 6X His tag in transfected human HEK293 cells by Immunocytochemistry/ Immunofluorescence incubated for 12 hours at 22°C. PFA fixed. Permeabilized using 0.1% Triton X-100 in PBS. Blocked using 3% BSA for 1 hour at 22°C. Secondary used at a 1/1000 dilution polyclonal Goat anti-mouse IgG (H+L) conjugated to Alexa Fluor 488 (green). DAPI staining (blue).

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• 

  Immunoprecipitation - Anti-6X His tag® antibody [HIS.H8] (ab18184)This image is courtesy of an Abreview submitted by Mr Chris Tracy
  Immunoprecipitation of His/FLAG tagged PDCD5 using ab18184 at 1/100 dilution.
  
  Lane 1 : MW Ladder (from top 120, 85, 50, 34, 25, 19 kDa)
  Lanes 2-5 : Immunoprecipitation of His/FLAG tagged PDCD5 using ab18184 from mixture of ~50 ng purified His/FLAG tagged PDCD5 and 15 µL RRL in 300 µL buffer.
  Lanes 6-7 : Purified PDCD5 from E. coli with ~60-70 ng protein.
  
  The lowest band in each lane is ~ 20 kDa and is PDCD5. The upper bands in lanes 2-5 are the heavy chain (~50 kDa, runs as doublet) and light chain (~25 or 26 kDa).
  
  Western Blot was performed with a mouse anti-FLAG primary antibody at 1/2000 dilution. An IRDye®800CW-conjugated goat anti-mouse IgG was used as a secondary antibody.

  See Abreview