Anti-Ctip2 antibody [25B6] - ChIP Grade (ab18465)
- Product nameAnti-Ctip2 antibody [25B6] - ChIP GradeSee all Ctip2 primary antibodies ...
- DescriptionRat monoclonal [25B6] to Ctip2 - ChIP Grade
- SpecificityDetects 2 bands representing Ctip2 at about 120kD. Ctip2 is highly expressed in brain and in malignant T-cell lines derived from patients with adult T-cell leukemia/lymphoma.
- Tested applicationsICC/IF, IHC-FoFr, WB, IP, IHC-P, IHC-Fr, ChIP, IHC-FrFl, Flow Cyt more details
- Species reactivityReacts with: Mouse, Guinea pig, Human, Zebrafish
Fusion protein (Human).
- EpitopeBetween amino acids 1-150 of CTIP2.
- Positive controlWB: nuclear extract from Jurkat cells immunoprecipitated with anti-Sir2 antibody IHC: adult mouse hippocampus, Adult mouse spinal cord ICC: Neonatal mouse hippocampal cultured neurons
- General notesHybridoma produced by fusion of a rat lymphocyte and mouse myeloma.
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 150mM Sodium chloride, 15mM HEPES, pH 7.5
- Concentration information loading...
- PurityIgG fraction
- Clonality Monoclonal
- Clone number25B6
Our Abpromise guarantee covers the use of ab18465 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||IHC-FoFr: Use at an assay dependent dilution. PubMed: 18523013|
|WB||WB: Use at an assay dependent dilution. Detects a band of approximately 120 kDa (predicted molecular weight: 95 kDa).|
|IP||IP: Use at an assay dependent dilution.|
|ChIP||ChIP: Use at an assay dependent dilution.|
|IHC-FrFl||IHC-FrFl: Use at an assay dependent concentration.|
|Flow Cyt||Flow Cyt: Use 1µg for 106 cells.|
- FunctionTumor-suppressor protein involved in T-cell lymphomas. May function on the P53-signaling pathway. May be a key regulator of both differentiation and survival during thymocyte development. Repress transcription through direct, TFCOUP2-independent binding to a GC-rich response element.
- Tissue specificityHighly expressed in brain and in malignant T-cell lines derived from patients with adult T-cell leukemia/lymphoma.
- Sequence similaritiesContains 6 C2H2-type zinc fingers.
- Cellular localizationNucleus.
- B cell lymphoma/leukemia 11B antibodyB-cell CLL/Lymphoma 11B antibodyB-cell lymphoma/leukemia 11B antibody
- BC11B_HUMAN antibodyBCL-11B antibodyBcl11B antibodyCOUP-TF interacting protein 2 antibodyCOUP-TF-interacting protein 2 antibodyCtip 2 antibodyhRit1 antibodyRadiation induced tumor suppressor gene 1 antibodyRadiation induced tumor suppressor gene 1 protein antibodyRadiation-induced tumor suppressor gene 1 protein antibodyRit 1 antibodyRit1 antibody
Anti-Ctip2 antibody [25B6] - ChIP Grade images
ab18465 staining Ctip2 in mouse brain tissue sections by IHC-Fr (Frozen sections). Tissue samples were fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 22°C. The sample was incubated with primary antibody (1/500) at 4°C for 16 hours. An Alexa Fluor®488-conjugated Goat polyclonal to rat IgG (1/1000) was used as secondary antibody. Staining was improved with citrate antigen retrieval.
Neonatal Mouse Hippocampal Neurons (Harvested at P1, grown 5d in culture on glial cell feeder layer).
Red is beta tubulin staining.
Green is ab18465.
Immunohistochemistry using ab18465 on Adult Mouse Hippocampus.
Neonatal mouse hippocampal neurons stained with ab18465 (top panel - green) and Ctip1 antibody (middle - red). Bottom panel is overlay of ab18465 and Ctip1 antibody staining - yellow indicates co-localisation, green is ab18465 alone and red is Ctip1 antibody alone.
Predicted band size : 95 kDa
Western blot using ab18465 on nuclear extract from Jurkat cells immunoprecipitated with anti-Sir2 antibody. Two bands are seen which may correspond to two CTIP2 transcripts present in Jurkat cells as previously reported (Bernard et al. 2001).
All lanes : Anti-Ctip2 antibody [25B6] - ChIP Grade (ab18465) at 1/500 dilution
Lane 1 : Mouse brain tissue lysate at 1.5 µg
Lane 2 : Mouse brain tissue lysate at 1.5 µg
Lane 3 : Mouse brain tissue lysate at 3 µg
IRDYE 680-conjugated Donkey Anti-Rat polyclonal. at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 95 kDa
Observed band size : 100,110 kDa (why is the actual band size different from the predicted?)
Exposure time : 10 minutes
This image is courtesy of an Anonymous Abreview.
ab18465 staining Ctip2 in Guinea Pig Brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% BSA for 30 minutes at 22°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/500 in blocking buffer) for 16 hours at 4°C. A Biotin-conjugated Goat anti-rat IgG polyclonal (1/200) was used as the secondary antibody.
Overlay histogram showing Jurkat cells stained with ab18465 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18465, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG (2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
References for Anti-Ctip2 antibody [25B6] - ChIP Grade (ab18465)
This product has been referenced in:
- Alsiö JM et al. Ikaros promotes early-born neuronal fates in the cerebral cortex. Proc Natl Acad Sci U S A 110:E716-25 (2013). ICC/IF ; Mouse . Read more (PubMed: 23382203) »
- Hodge RD et al. Tbr2 expression in Cajal-Retzius cells and intermediate neuronal progenitors is required for morphogenesis of the dentate gyrus. J Neurosci 33:4165-80 (2013). Read more (PubMed: 23447624) »