Anti-Kv2.1/KCNB1 antibody [K89/34] (ab192761)
Key features and details
- Mouse monoclonal [K89/34] to Kv2.1/KCNB1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
-
Product name
Anti-Kv2.1/KCNB1 antibody [K89/34]
See all Kv2.1/KCNB1 primary antibodies -
Description
Mouse monoclonal [K89/34] to Kv2.1/KCNB1 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- This antibody gave a positive result in IHC in the following FFPE tissue: Human normal Hippocampus
-
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading...
-
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
K89/34 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab192761 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 98 kDa (predicted molecular weight: 95 kDa).
|
|
IHC-P |
Use a concentration of 1 µg/ml.
|
Notes |
---|
WB
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 98 kDa (predicted molecular weight: 95 kDa). |
IHC-P
Use a concentration of 1 µg/ml. |
Target
-
Function
Mediates the voltage-dependent potassium ion permeability of excitable membranes. Channels open or close in response to the voltage difference across the membrane, letting potassium ions pass in accordance with their electrochemical gradient. -
Sequence similarities
Belongs to the potassium channel family. B (Shab) (TC 1.A.1.2) subfamily. Kv2.1/KCNB1 sub-subfamily. -
Domain
The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
The tail may be important in modulation of channel activity and/or targeting of the channel to specific subcellular compartments. -
Post-translational
modificationsHighly phosphorylated on serine residues in the C-terminal. Differential phosphorylation on a subset of serines allows graded activity-dependent regulation of channel gating. Phosphorylation on Ser-457, Ser-541, Ser-567, Ser-607, Ser-656 and Ser-720 as well as the N-terminal Ser-15 are all regulated by calcineurin-mediated dephosphorylation. Particularly, Ser-607 and Tyr-128 are significant sites of voltage-gated regulation through phosphorylation/ dephosphorylation activities. Tyr-128 can be dephosphorylated by PTPalpha and cyt-PTPepsilon. Phosphorylation levels on Ser-607 are supersensitive to neuronal activity. Phosphorylation on Ser-567 is reduced during postnatal development with low levels at P2 and P5. Levels then increase to reach adult levels by P14. Phosphorylation levels on Ser-564 and Ser-607 are greatly reduced during seizures, by 40% and 85% respectively. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 3745 Human
- Entrez Gene: 16500 Mouse
- Entrez Gene: 25736 Rat
- Omim: 600397 Human
- SwissProt: Q14721 Human
- SwissProt: Q03717 Mouse
- SwissProt: P15387 Rat
- Unigene: 84244 Human
see all -
Alternative names
- Delayed rectifier potassium channel 1 antibody
- Delayed rectifier potassium channel Kv2.1 antibody
- DRK 1 antibody
see all
Images
-
IHC image of Kv2.1/KCNB1 staining in Human Normal Hippocampus formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab192761, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
-
All lanes : Anti-Kv2.1/KCNB1 antibody [K89/34] (ab192761) at 1 µg/ml
Lane 1 : Rat brain normal tissue lysate - total protein (ab29475)
Lane 2 : Hippocampus (Mouse) Tissue Lysate
Lane 3 : Human brain tissue lysate - total protein (ab29466)
Lane 4 : Rat Olfactory Bulb Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 95 kDa
Observed band size: 98 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
Datasheets and documents
-
SDS download
-
Datasheet download
References (5)
ab192761 has been referenced in 5 publications.
- Gao J et al. Voltage-gated potassium channels are involved in oxymatrine-regulated islet function in rat islet ß cells and INS-1 cells. Iran J Basic Med Sci 24:460-468 (2021). PubMed: 34094027
- Xiong J et al. Correlation Analyses of Clinical Manifestations and Variant Effects in KCNB1-Related Neurodevelopmental Disorder. Front Pediatr 9:755344 (2021). PubMed: 35071126
- Lee MC et al. Human Labor Pain Is Influenced by the Voltage-Gated Potassium Channel KV6.4 Subunit. Cell Rep 32:107941 (2020). PubMed: 32697988
- Schulien AJ et al. Targeted disruption of Kv2.1-VAPA association provides neuroprotection against ischemic stroke in mice by declustering Kv2.1 channels. Sci Adv 6:N/A (2020). PubMed: 32937450
- Corona C et al. Activating Transcription Factor 4 (ATF4) Regulates Neuronal Activity by Controlling GABABR Trafficking. J Neurosci 38:6102-6113 (2018). PubMed: 29875265