Recombinant Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16312] to KCTD5 - C-terminal
- Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-KCTD5 antibody [EPR16312] - C-terminal
See all KCTD5 primary antibodies -
Description
Rabbit monoclonal [EPR16312] to KCTD5 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, Jurkat, RAW 264.7, C6 and NIH 3T3 whole cell lysates; Human kidney and Rat liver tissues; A431 cells; Jurkat cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16312 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab194825 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/70.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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ICC/IF |
1/100.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
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Notes |
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Flow Cyt (Intra)
1/70. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ICC/IF
1/100. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). |
Target
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Function
Its interaction with CUL3 suggests that it may act as a substrate adapter in some E3 ligase complex. Does not affect the function of Kv channel Kv2.1/KCNB1, Kv1.2/KCNA2, Kv4.2/KCND2 and Kv3.4/KCNC4. -
Sequence similarities
Contains 1 BTB (POZ) domain. -
Domain
The BTB (POZ) domain is atypical and mediates the formation of a homopentamer instead of a homotetramer. Homopentamerization is due to the presence of 4 residues in the BTB (POZ) domain: Leu-56, Gly-100, Val-112 and Ala-118. -
Cellular localization
Cytoplasm > cytosol. Nucleus. Predominantly cytoplasmic, translocated to the nucleus upon interaction with Rep proteins. - Information by UniProt
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Database links
- Entrez Gene: 54442 Human
- Entrez Gene: 69259 Mouse
- Entrez Gene: 287109 Rat
- Omim: 611285 Human
- SwissProt: Q9NXV2 Human
- SwissProt: Q8VC57 Mouse
- SwissProt: B5DEL1 Rat
- Unigene: 61960 Human
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Alternative names
- BTB/POZ domain-containing protein KCTD5 antibody
- FLJ20040 antibody
- KCTD5 antibody
see all
Images
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All lanes : Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825) at 1/1000 dilution
Lane 1 : Human embryonic kidney epithelial cell (HEK293T) whole cell lysate
Lane 2 : Human embryonic kidney epithelial cell (HEK293T) transfected with KCTD5 expression vector containing a His-tag whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 26 kDa
Exposure time: 1 secondBlocking and diluting buffer and concentration: 5% NFDM /TBST.
ab181602 was used as a GAPDH loading control.
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All lanes : Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825) at 1/1000 dilution
Lane 1 : HeLa whole cell lysates
Lane 2 : Jurkat whole cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 26 kDa -
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling KCTD5 with ab194825 at 1/100 dilution (3.8 μg/ml). A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 was used as secondary. Counterstain: Hematoxylin.
Inset image: negative control obtained using PBS instead of ab194825.
Note: Both cytoplasm and nuclear staining on human kidney tissue.Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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All lanes : Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825) at 1/1000 dilution
Lane 1 : C6 whole cell lysates
Lane 2 : RAW 264.7 whole cell lysates
Lane 3 : NIH 3T3 whole cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 26 kDa -
Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling KCTD5 with ab194825 at 1/100 dilution (3.8 μg/ml). A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 was used as secondary. Counterstain: Hematoxylin.
Inset image: negative control obtained using PBS instead of ab194825.
Note: Both cytoplasm and nuclear staining on rat liver tissue.Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A431 cells labeling KCTD5 with ab194825 at 1/100 dilution (3.8 μg/ml), followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Counterstained with Dapi (blue). Both cytoplasm and nuclear staining on A431 cell line is observed.
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Intracellular flow cytometric analysis of Jurkat cells labeling KCTD5 with ab194825 at 1/70 dilution (Red). A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Cells without incubation with primary antibody and secondary antibody (Blue). Rabbit monoclonal IgG was used as isotype control (Black).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab194825 has not yet been referenced specifically in any publications.