HRP Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5] (ab197968)
Key features and details
- HRP Mouse monoclonal [16D10AD9AH5] to Ubiquinol-Cytochrome C Reductase Core Protein I
- Suitable for: WB, IHC-P
- Reacts with: Human
- Conjugation: HRP
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
HRP Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5]
See all Ubiquinol-Cytochrome C Reductase Core Protein I primary antibodies -
Description
HRP Mouse monoclonal [16D10AD9AH5] to Ubiquinol-Cytochrome C Reductase Core Protein I -
Host species
Mouse -
Conjugation
HRP -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Full length native protein (purified) corresponding to Cow Ubiquinol-Cytochrome C Reductase Core Protein I.
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Positive control
- WB: Human heart mitochondrial lysate and HepG2 whole cell lysate. IHC-P: Normal human colon tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
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Purity
IgG fraction -
Purification notes
ab197968 is produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
Clonality
Monoclonal -
Clone number
16D10AD9AH5 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab197968 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/5000. Detects a band of approximately 49 kDa (predicted molecular weight: 53 kDa).
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IHC-P |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/5000. Detects a band of approximately 49 kDa (predicted molecular weight: 53 kDa). |
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. This protein may mediate formation of the complex between cytochromes c and c1. -
Sequence similarities
Belongs to the peptidase M16 family. UQCRC1/QCR1 subfamily. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 282393 Cow
- Entrez Gene: 7384 Human
- Entrez Gene: 22273 Mouse
- Entrez Gene: 301011 Rat
- Omim: 191328 Human
- SwissProt: P31800 Cow
- SwissProt: P31930 Human
- SwissProt: Q8K2S8 Mouse
see all -
Alternative names
- Complex III subunit 1 antibody
- Core protein I antibody
- Cytochrome b c1 complex subunit 1, mitochondrial antibody
see all
Images
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HRP Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5] (ab197968) at 1/5000 dilution + Human heart tissue lysate - mitochondrial extract (ab110337) at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 53 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Exposure time: 18 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab197968 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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HRP Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5] (ab197968) at 1/5000 dilution + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 20 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 53 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab197968 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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IHC image of Ubiquinol-Cytochrome C Reductase Core Protein I staining in a section of formalin-fixed paraffin-embedded normal human colon*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab197968, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab197968 has not yet been referenced specifically in any publications.