Immunocytochemistry/ Immunofluorescence abreview for Anti-Oct4 antibody - ChIP Grade

Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (embryonic stem cell (HUES4))
Specification
embryonic stem cell (HUES4)
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% triton
Blocking step
Dako Protein Block (0.25% caesin) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.25% · Temperature: 25°C

Other product details

Dilution
1/200
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Dako Diluent

Secondary antibody

Name
Non-Abcam antibody was used: Jackson 111-095-003
Host species: Goat
Clonality: Polyclonal
Conjugation: Fluorescein
Dilution
1/500

Additional data

Additional Notes
Fixation: 4% paraformaldehyde in PBS, 20 min, 25 C.
Permeabilization: 0.1% triton-x in PBS, 15 min, 25 C.
Human embryonic stem cells (HUES4) were cultured on matrigel coated dishes in Nutristem medium to maintain pluripotency. The undifferentiated embryonic stem cells are positive for the stem cell transcription factor OCT4 (ab19857 in green). Dapi staining is shown in blue. Magnification of top panels: 100x, bottom panels: 200x. Negative controls of fluorescently conjugated secondary antibodies only showed no staining.
Username

Ms. Emily Moorefield

Verified customer

Submitted Feb 24 2011

Sign up