Anti-AKAP12 antibody - C-terminal (ab198895)
Key features and details
- Rabbit polyclonal to AKAP12 - C-terminal
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-AKAP12 antibody - C-terminal
See all AKAP12 primary antibodies -
Description
Rabbit polyclonal to AKAP12 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human AKAP12 (C terminal). Gene Accession: NP_005091.
Database link: Q02952 -
Positive control
- HT29 cell lysate and Human brain tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab198895 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/200.
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WB |
1/200 - 1/1000. Predicted molecular weight: 191 kDa.
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Notes |
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IHC-P
1/50 - 1/200. |
WB
1/200 - 1/1000. Predicted molecular weight: 191 kDa. |
Target
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Function
Anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC). -
Tissue specificity
Expressed in endothelial cells, cultured fibroblasts and osteosarcoma, but not in platelets, leukocytes, monocytic cell lines or peripherical blood cells. -
Sequence similarities
Contains 3 AKAP domains. -
Domain
Polybasic regions located between residues 266 and 557 are involved in binding PKC. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. May be part of the cortical cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 9590 Human
- Omim: 604698 Human
- SwissProt: Q02952 Human
- Unigene: 371240 Human
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Alternative names
- A kinase (PRKA) anchor protein (gravin) 12 antibody
- A kinase Anchor Protein 12 antibody
- A kinase anchor protein 250kDa antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab198895 has been referenced in 2 publications.
- Jiang F et al. Construction of mRNA prognosis signature associated with differentially expressed genes in early stage of stomach adenocarcinomas based on TCGA and GEO datasets. Eur J Med Res 27:205 (2022). PubMed: 36253873
- Chang J et al. MiR-338-3p improved lung adenocarcinoma by AKAP12 suppression. Arch Med Sci 17:462-473 (2021). PubMed: 33747281