Recombinant Anti-GEN1 antibody [EPR16863] (ab198989)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16863] to GEN1
- Suitable for: WB
- Reacts with: Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-GEN1 antibody [EPR16863]
See all GEN1 primary antibodies -
Description
Rabbit monoclonal [EPR16863] to GEN1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, 293, SH-SY5Y, MDA-MB-435S and C6 whole cell lysates; rat kidney tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16863 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab198989 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa).
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Notes |
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WB
1/1000. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa). |
Target
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Function
Endonuclease which cleaves flap structures at the junction between single-stranded DNA and double-stranded DNA. Specific for 5'-overhanging flap structures in which the 5'-upstream of the flap is completely double-stranded. Prefers the blocked-flap structures similar to those occurring at replication forks, in which the 5' single-strand overhang of the flap is double-stranded. -
Sequence similarities
Belongs to the XPG/RAD2 endonuclease family. GEN subfamily. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 348654 Human
- Entrez Gene: 298884 Rat
- Omim: 612449 Human
- SwissProt: Q17RS7 Human
- Unigene: 467793 Human
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Alternative names
- DKFZp781F0986 antibody
- Flap endonuclease GEN homolog 1 antibody
- FLJ40869 antibody
see all
Images
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All lanes : Anti-GEN1 antibody [EPR16863] (ab198989) at 1/1000 dilution
Lane 1 : Rat kidney tissue lysate
Lane 2 : C6 (rat glial tumor cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution
Predicted band size: 103 kDa
Observed band size: 103 kDaBlocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 3 minutes
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All lanes : Anti-GEN1 antibody [EPR16863] (ab198989) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : 293 (human embryonic kidney) whole cell lysate
Lane 3 : SH-SY5Y (human neuroblastoma cell line from bone marrow)
Lane 4 : MDA-MB-435S (human ductal carcinoma cell line)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution
Predicted band size: 103 kDa
Observed band size: 103 kDaBlocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 1 minute
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab198989 has not yet been referenced specifically in any publications.