Anti-beta Actin antibody [mAbcam 8226] (HRP) - Loading Control (ab20272)
Overview
- Product nameAnti-beta Actin antibody [mAbcam 8226] (HRP) - Loading ControlSee all beta Actin primary antibodies ...
- DescriptionMouse monoclonal [mAbcam 8226] to beta Actin - Loading Control (HRP)
- ConjugationHRP
- Tested applicationsWB more details
- Species reactivityReacts with: Mouse, Rat, Rabbit, Chicken, Cow, Dog, Human, Pig, Fruit fly (Drosophila melanogaster), African Green Monkey, Chinese Hamster
Predicted to work with: Sheep, Horse, Guinea pig - Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human beta Actin.
- Positive controlHeLa whole cell lysate, 3T3 whole cell lysate, Rat brain lysate
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: 50% Glycerol, PBS, pH 7.4 -
Concentration information loading... - PurityIgG fraction
- Clonality Monoclonal
- Clone numbermAbcam 8226
- MyelomaSp2/0-Ag14
- IsotypeIgG1
- Light chain typekappa
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab20272 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/5000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).Can be blocked with beta Actin peptide (ab13772). Abcam strongly recommends blocking and incubation using 5% BSA as we have found that use of 5% milk seems to drastically reduce the intensity of the signal obtained. Please refer to WB image for further details. |
Target
- FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
- Involvement in diseaseDefects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
- Sequence similaritiesBelongs to the actin family.
- Post-translational
modificationsISGylated. - Cellular localizationCytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
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Database links
- Entrez Gene: 60 Human
- Entrez Gene: 11461 Mouse
- Entrez Gene: 100009272 Rabbit
- Entrez Gene: 81822 Rat
- Omim: 102630 Human
- SwissProt: P60706 Chicken
- SwissProt: P60712 Cow
- SwissProt: P60708 Horse
- SwissProt: P60709 Human
- SwissProt: P60710 Mouse
- SwissProt: P29751 Rabbit
- SwissProt: P60711 Rat
- SwissProt: P60713 Sheep
- Unigene: 520640 Human
- Unigene: 708120 Human
- Unigene: 727576 Human
- Unigene: 328431 Mouse
- Unigene: 391967 Mouse
- Unigene: 94978 Rat
see all
Target information above from: UniProt accession
P60709
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
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Alternative names
- A X actin like protein antibodyACTB antibodyACTB_HUMAN antibody
- Actin antibodyActin cytoplasmic 1 antibodyActx antibodyb-actin antibodyBeta cytoskeletal actin antibodyBeta-actin antibodycytoplasmic 1 antibodyMelanoma X actin antibodyN-terminally processed antibodyPS1TP5 binding protein 1 antibodyPS1TP5BP1 antibody
see all
Anti-beta Actin antibody [mAbcam 8226] (HRP) - Loading Control images
-
![Western blot - beta Actin antibody [mAbcam 8226] - Loading Control (ab20272)](http://a.abcam.com/ps/datasheet/Images/20/ab20272/ab20272 datasheet 2 copy.jpg)
Western blot - beta Actin antibody [mAbcam 8226] - Loading Control (ab20272)All lanes : Anti-beta Actin antibody [mAbcam 8226] (HRP) - Loading Control (ab20272) at 1/5000 dilution
Lane 1 : human testis lysate
Lane 2 : human brain lysate
Lane 3 : human tonsil lysate
Lane 4 : mouse brain lysate
Lane 5 : mouse spleen lysate
Lysates/proteins at 20 µg per lane.
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 42 kDa
Exposure time : 2 minutes -
![Western blot - beta Actin antibody [mAbcam 8226] - Loading Control (ab20272)](http://a.abcam.com/ps/datasheet/Images/20/ab20272/ab20272_1.jpg)
Western blot - beta Actin antibody [mAbcam 8226] - Loading Control (ab20272)All lanes : Anti-beta Actin antibody [mAbcam 8226] (HRP) - Loading Control (ab20272) at 1/5000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : 3T3 whole cell lysate
Lane 3 : Rat brain lysate
Lysates/proteins at 20 µg per lane.
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 42 kDa
Observed band size : 42 kDa
Exposure time : 30 seconds -
![Western blot - beta Actin antibody [mAbcam 8226] (HRP) - Loading Control (ab20272)](http://a.abcam.com/ps/datasheet/images/20/ab20272/beta-Actin-Primary-antibodies-ab20272-2.jpg)
Western blot - beta Actin antibody [mAbcam 8226] (HRP) - Loading Control (ab20272)Lanes 1 - 4 : Anti-beta Actin antibody [mAbcam 8226] (HRP) - Loading Control (ab20272) at 1/5000 dilution (Blocked in 5% BSA)
Lanes 5 - 8 : Anti-beta Actin antibody [mAbcam 8226] (HRP) - Loading Control (ab20272) at 1/5000 dilution (Blocked in 5% MILK)
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 :NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)
Lane 4 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 6 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 7 :NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)
Lane 8 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 42 kDa
Observed band size : 42 kDa
Exposure time : 1 minute
References for Anti-beta Actin antibody [mAbcam 8226] (HRP) - Loading Control (ab20272)
This product has been referenced in:
- Spindler SR et al. Novel protein kinase signaling systems regulating lifespan identified by small molecule library screening using Drosophila. PLoS One 7:e29782 (2012). WB ; Fruit fly (Drosophila melanogaster) . Read more (PubMed: 22363408) »
- Zimnicka AM et al. Acquisition of dietary copper: a role for anion transporters in intestinal apical copper uptake. Am J Physiol Cell Physiol 300:C588-99 (2011). Read more (PubMed: 21191107) »
