Anti-BDNF antibody [3C11] (ab203573)
Key features and details
- Mouse monoclonal [3C11] to BDNF
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human, Chinese hamster
- Isotype: IgG1
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-BDNF antibody [3C11]
See all BDNF primary antibodies -
Description
Mouse monoclonal [3C11] to BDNF -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Chinese hamster -
Immunogen
Recombinant full length protein corresponding to Human BDNF aa 100 to the C-terminus. Full length mature form without signal peptide or propeptide. Produced in E coli.
Database link: P23560 -
Positive control
- Recombinant human BDNF; CHO supernatant containing BDNF; Rat hippocampal neuron culture lysate; proBDNF-expressing U2OS cells, brain tissue lysate. IHC-P: FFPE Human Hippocampus Normal
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General notes
For BDNF, multiple WB bands are possible and expected. The human protein has 5 isoforms (precursors: 28 – 37 kDa) and can be glycosylated (Uniprot: http://www.uniprot.org/uniprot/P23560). The mature form is expected at ~14 kDa (monomer) and the dimer at ~28 kDa.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
3C11 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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ELISA pair antibody
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab203573 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 15 kDa.
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ICC/IF |
Use a concentration of 2 - 20 µg/ml.
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Notes |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 15 kDa. |
ICC/IF
Use a concentration of 2 - 20 µg/ml. |
Target
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Function
During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. -
Tissue specificity
Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta. -
Involvement in disease
Bulimia nervosa 2
Congenital central hypoventilation syndrome -
Sequence similarities
Belongs to the NGF-beta family. -
Post-translational
modificationsThe propeptide is N-glycosylated and glycosulfated.
Converted into mature BDNF by plasmin (PLG). -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 627 Human
- Entrez Gene: 12064 Mouse
- Entrez Gene: 24225 Rat
- Omim: 113505 Human
- SwissProt: P23560 Human
- SwissProt: P21237 Mouse
- SwissProt: P23363 Rat
- Unigene: 502182 Human
see all -
Alternative names
- Abrineurin antibody
- ANON2 antibody
- BDNF antibody
see all
Images
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Anti-BDNF antibody [3C11] (ab203573) at 5 µg/ml + CHO supernatant containing BDNF at 10 µl
Secondary
HRP-conjugated goat anti-mouse
Predicted band size: 15 kDa -
IHC image of BDNF staining in Normal Human Hippocampus formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab203573, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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All lanes : Anti-BDNF antibody [3C11] (ab203573) at 5 µg/ml
Lane 1 : Human hippocampus
Lane 2 : Rat hippocampus
Lane 3 : Mouse hippocampus
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : goat anti-mouse IgG IR-680 (green) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 15 kDa
Additional bands at: 15 kDa (possible mature (processed) protein), 40 kDa (possible immature (unprocessed))This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab203573 (1/1000) overnight at 4°C. Antibody binding was detected using goat anti-mouse IgG IR-680 (green) at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx
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Immunofluorescent analysis of proBDNF expressing U2OS cells labeling BDNF with ab203573 at 10 µg/mL (Left hand panel). Goat anti-mouse AlexaFluor488 was used as secondary antibody. For nuclear staining DAPI was used. Right hand panel: Negative control (non-transfected U2OS cells).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (40)
ab203573 has been referenced in 40 publications.
- Bankole O et al. Beneficial and Sexually Dimorphic Response to Combined HDAC Inhibitor Valproate and AMPK/SIRT1 Pathway Activator Resveratrol in the Treatment of ALS Mice. Int J Mol Sci 23:N/A (2022). PubMed: 35162978
- Shi HJ et al. Requirement of hippocampal DG nNOS-CAPON dissociation for the anxiolytic and antidepressant effects of fluoxetine. Theranostics 12:3656-3675 (2022). PubMed: 35664081
- Leem YH et al. Neurogenic effects of rotarod walking exercise in subventricular zone, subgranular zone, and substantia nigra in MPTP-induced Parkinson's disease mice. Sci Rep 12:10544 (2022). PubMed: 35732806
- Sadeghi MA et al. Chronically altered NMDAR signaling in epilepsy mediates comorbid depression. Acta Neuropathol Commun 9:53 (2021). PubMed: 33762011
- Pawar G et al. Endonasal CNS Delivery System for Blood-Brain Barrier Impermeant Therapeutic Oligonucleotides Using Heterotopic Mucosal Engrafting. Front Pharmacol 12:660841 (2021). PubMed: 33953687