Anti-HLA DP antibody [B7/21] (ab20897)

Overview

• Product nameAnti-HLA DP antibody [B7/21]See all HLA DP primary antibodies ...
• Description
  Mouse monoclonal [B7/21] to HLA DP
• SpecificityRecognizes a (Mr 26-34 kDa) class II major histocompatibility complex (MHC) antigen. Recognizes a monomorphic determinant present on cells expressing DP1, DP2, DP3, DP4, and DP5. The HLA-DP antigen is present on approximately 10% of normal peripheral blood lymphocytes but not on resting peripheral T-lymphocytes. HLA-DP is present in low density on peripheral blood monocytes and mitogen-stimulated T-lymphoblasts. Anti-HLA-DP reacts with most B-cell lines.
• Tested applicationsFlow Cyt, Inhibition Assay more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  The details of the immunogen for this antibody are not available.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: 1% BSA, 0.15M Sodium chloride, 0.01M Potassium phosphate. pH 7.2
• Concentration information loading...
• PurityProtein G purified
• Clonality Monoclonal
• Clone numberB7/21
• Myelomaunknown
• IsotypeIgG3
• Light chain typeunknown
• Research Areas
  • Immunology
  • Adaptive Immunity
  • MHC
  • Class II

Applications

Target

• RelevanceHLA-DPA1 belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta (DPB) chain, both anchored in the membrane. It plays a vital role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (B lymphocytes, dendritic cells and macrophages). Within the DP molecule the alpha and beta chain contain the polymorphisms governing the peptide binding specificities, resulting in up to 4 different molecules.
• Cellular localizationType I membrane protein.
• Database links
  • Entrez Gene: 3113 Human
  • Entrez Gene: 3113 Human
  • Omim: 142880 Human
  • SwissProt: P20036 Human
  • SwissProt: P20036 Human
  • Unigene: 347270 Human

• Alternative names
  DP(W3) antibodyDP(W4) antibodyHLA class II histocompatibility antigen DP alpha 1 chain antibody

  HLA class II histocompatibility antigen DP alpha chain antibodyHLA class II histocompatibility antigen DP antibodyHLA DP1A antibodyHLA DPA 1 antibodyHLA SB alpha chain antibodyHLA SB antibodyHLADP antibodyHLADPA1 antibodyHLADPB1 antibodyHLASB antibodyHLASB histocompatibility type antibodyHuman MHC class II HLA SB alpha gene antibodyMajor histocompatibility complex class II DP alpha 1 antibodyMHC class II antigen antibodyMHC class II DP3 alpha antibodyMHC class II DPA1 antibodyMHC class II HLA DPA1 antigen antibodyMHC DPB1 antibodyOTTHUMP00000062983 antibodyPLT1 antibodyPrimed lymphocyte test 1 antibodySB2beta antibody

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Anti-HLA DP antibody [B7/21] images

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  Flow Cytometry-HLA DP antibody [B7/21](ab20897)
  Overlay histogram showing RAMOS cells stained with ab20897(red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab20897, 1µg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG3 [MG3-35](ab18394, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.
  Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.