Anti-HLA DP antibody [B7/21] (ab20897)
- Product nameAnti-HLA DP antibody [B7/21]See all HLA DP primary antibodies ...
- DescriptionMouse monoclonal [B7/21] to HLA DP
- SpecificityRecognizes a (Mr 26-34 kDa) class II major histocompatibility complex (MHC) antigen. Recognizes a monomorphic determinant present on cells expressing DP1, DP2, DP3, DP4, and DP5. The HLA-DP antigen is present on approximately 10% of normal peripheral blood lymphocytes but not on resting peripheral T-lymphocytes. HLA-DP is present in low density on peripheral blood monocytes and mitogen-stimulated T-lymphoblasts. Anti-HLA-DP reacts with most B-cell lines.
- Tested applicationsFlow Cyt, Inhibition Assay more details
- Species reactivityReacts with: Human
The details of the immunogen for this antibody are not available.
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.09% Sodium Azide
Constituents: 1% BSA, 0.15M Sodium chloride, 0.01M Potassium phosphate. pH 7.2
- Concentration information loading...
- PurityProtein G purified
- Clonality Monoclonal
- Clone numberB7/21
- Light chain typeunknown
- Research Areas
Our Abpromise guarantee covers the use of ab20897 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Flow Cyt: Use 1µg for 106 cells.|
|Inhibition Assay||Inhib: Use at an assay dependent dilution.|
- RelevanceHLA-DPA1 belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta (DPB) chain, both anchored in the membrane. It plays a vital role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (B lymphocytes, dendritic cells and macrophages). Within the DP molecule the alpha and beta chain contain the polymorphisms governing the peptide binding specificities, resulting in up to 4 different molecules.
- Cellular localizationType I membrane protein.
- DP(W3) antibodyDP(W4) antibodyHLA class II histocompatibility antigen DP alpha 1 chain antibody
- HLA class II histocompatibility antigen DP alpha chain antibodyHLA class II histocompatibility antigen DP antibodyHLA DP1A antibodyHLA DPA 1 antibodyHLA SB alpha chain antibodyHLA SB antibodyHLADP antibodyHLADPA1 antibodyHLADPB1 antibodyHLASB antibodyHLASB histocompatibility type antibodyHuman MHC class II HLA SB alpha gene antibodyMajor histocompatibility complex class II DP alpha 1 antibodyMHC class II antigen antibodyMHC class II DP3 alpha antibodyMHC class II DPA1 antibodyMHC class II HLA DPA1 antigen antibodyMHC DPB1 antibodyOTTHUMP00000062983 antibodyPLT1 antibodyPrimed lymphocyte test 1 antibodySB2beta antibody
Anti-HLA DP antibody [B7/21] images
Overlay histogram showing RAMOS cells stained with ab20897(red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab20897, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG3 [MG3-35](ab18394, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
References for Anti-HLA DP antibody [B7/21] (ab20897)
This product has been referenced in:
- Tanaka M et al. Induction of HLA-DP4-Restricted Anti-Survivin Th1 and Th2 Responses Using an Artificial Antigen-Presenting Cell. Clin Cancer Res 17:5392-401 (2011). Read more (PubMed: 21705450) »
- Wang M et al. HLA class I binding 9mer peptides from influenza A virus induce CD4 T cell responses. PLoS One 5:e10533 (2010). Measuring secreted proteins from cells . Read more (PubMed: 20479886) »