Overview
- Product nameAnti-Cyclin B1 antibodySee all Cyclin B1 primary antibodies ...
- DescriptionRabbit polyclonal to Cyclin B1
- SpecificityAntiserum will specifically react with a 62 kDa Cyclin B1 protein. No reaction was observed against other related cyclins.
- Tested applicationsWB, IP, ELISA more details
- Species reactivityReacts with: Mouse, Rat, Human
- Immunogen
Fusion protein (Human).
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.01% Sodium Azide
- PurityWhole antiserum
- Purification notesThis product was prepared from monospecific antiserum by delipidation and defibrination.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab2096 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 48 kDa). |
| IP | IP: Use at an assay dependent dilution. |
| ELISA | ELISA: 1/500 - 1/2000. |
Target
- FunctionEssential for the control of the cell cycle at the G2/M (mitosis) transition.
- Sequence similaritiesBelongs to the cyclin family. Cyclin AB subfamily.
- Developmental stageAccumulates steadily during G2 and is abruptly destroyed at mitosis.
- Post-translational
modificationsUbiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases. - Cellular localizationCytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.
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Database links
- Entrez Gene: 891 Human
- Entrez Gene: 268697 Mouse
- Entrez Gene: 25203 Rat
- Omim: 123836 Human
- SwissProt: P14635 Human
- SwissProt: P24860 Mouse
- SwissProt: P30277 Rat
- Unigene: 23960 Human
- Unigene: 260114 Mouse
- Unigene: 380027 Mouse
- Unigene: 482545 Mouse
- Unigene: 9232 Rat
see all
Target information above from: UniProt accession
P14635
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- CCNB 1 antibodyCCNB antibodyCCNB1 antibody
- CCNB1_HUMAN antibodyG2 mitotic specific cyclin B1 antibodyG2/mitotic-specific cyclin-B1 antibody
see all
References for Anti-Cyclin B1 antibody (ab2096)
This product has been referenced in:
- Kotecha M et al. Inhibition of DNA binding of the NF-Y transcription factor by the pyrrolobenzodiazepine-polyamide conjugate GWL-78. Mol Cancer Ther 7:1319-28 (2008). WB ; Mouse . Read more (PubMed: 18483319) »
- Morris C & Jalinot P Silencing of human Int-6 impairs mitosis progression and inhibits cyclin B-Cdk1 activation. Oncogene 24:1203-11 (2005). Read more (PubMed: 15558017) »
