Anti-alpha smooth muscle Actin antibody (ab21027)
Overview
- Product nameAnti-alpha smooth muscle Actin antibodySee all alpha smooth muscle Actin primary antibodies ...
- DescriptionGoat polyclonal to alpha smooth muscle Actin
- Tested applicationsWB, IHC-Fr more details
- Species reactivityReacts with: Mouse, Rat, Human, Pig
Predicted to work with: Dog - Immunogen
Synthetic peptide: EEEDSTALVC, corresponding to N terminal amino acids 3-12 of Human alpha smooth muscle Actin.
- Positive controlLysate prepared from human duodenum.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Clonality Polyclonal
- IsotypeIgG
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Research Areas
Applications
Our Abpromise guarantee covers the use of ab21027 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 42 kDa). |
| IHC-Fr | IHC-Fr: Use at an assay dependent concentration. PubMed: 20947685 |
Target
- FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
- Involvement in diseaseDefects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.
- Sequence similaritiesBelongs to the actin family.
- Cellular localizationCytoplasm > cytoskeleton.
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Database links
- Entrez Gene: 59 Human
- Entrez Gene: 11475 Mouse
- Entrez Gene: 733615 Pig
- Entrez Gene: 81633 Rat
- Omim: 102620 Human
- SwissProt: P62736 Human
- SwissProt: P62737 Mouse
- SwissProt: P62738 Rat
- Unigene: 500483 Human
- Unigene: 213025 Mouse
- Unigene: 195319 Rat
- Unigene: 3114 Rat
see all
Target information above from: UniProt accession
P62736
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- AAT6 antibodyACTA_HUMAN antibodyACTA2 antibody
- Actin alpha 2 smooth muscle aorta antibodyActin aortic smooth muscle antibodyActin vascular smooth muscle antibodyActin, aortic smooth muscle antibodyACTSA antibodyACTVS antibodyAlpha 2 actin antibodyAlpha actin 2 antibodyAlpha cardiac actin antibodyAlpha smooth muscle antibodyAlpha-actin 2 antibodyAlpha-actin-2 antibodyCell growth inhibiting gene 46 protein antibodyCell growth-inhibiting gene 46 protein antibodyGIG46 antibodyGrowth inhibiting gene 46 antibodyMYMY5 antibody
see all
Anti-alpha smooth muscle Actin antibody images
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Western blot - alpha smooth muscle Actin antibody (ab21027)Anti-alpha smooth muscle Actin antibody (ab21027) at 0.3 µg/ml + human duodenum lysate at 35 µg
developed using the ECL technique
Predicted band size : 42 kDa
Observed band size : 45 kDa (why is the actual band size different from the predicted?) -
Immunohistochemistry (Frozen sections) - Anti-alpha smooth muscle Actin antibody (ab21027)Image courtesy of an anonymous Abreview.ab21027 staining alpha smooth muscle Actin in porcine heart tissue by Immunohistochemistry (Frozen sections).Tissue was fixed in acetone, permeabilized using 0.3% Triton/PBS, blocked using 10% serum for 30 minutes at 22°C and then incubated with ab21027 at a 1/250 dilution for 16 hours at 4 °C. The secondary used was an FITC conjugated donkey anti-goat polyclonal used at a 1/1000 dilution.The green color indicates the staining of alpha smooth muscle actin. The blue color indicates staining of nuclei using DAPI. -
Western blot - Anti-alpha smooth muscle Actin antibody (ab21027)Lane 1 : Anti-alpha smooth muscle Actin antibody (ab21027) at 1 µg/ml
Lane 2 : Anti-alpha smooth muscle Actin antibody (ab21027) at 1 mg/ml
Lane 3 : anti-DYKDDDDK Tag at 1/3000 dilution
Lane 1 : HEK293 lysate over expressing Human ACTA2 with DYKDDDDK tag
Lane 2 : Mock-transfected HEK293 lysate
Lane 3 : HEK293 lysate over expressing Human ACTA2 with DYKDDDDK tag
Lysates/proteins at 10 µg per lane.
developed using the ECL technique
Predicted band size : 42 kDa
References for Anti-alpha smooth muscle Actin antibody (ab21027)
This product has been referenced in:
- van Gils JM et al. The neuroimmune guidance cue netrin-1 promotes atherosclerosis by inhibiting the emigration of macrophages from plaques. Nat Immunol 13:136-43 (2012). Read more (PubMed: 22231519) »
- Chang AY et al. Calcineurin mediates bladder wall remodeling secondary to partial outlet obstruction. Am J Physiol Renal Physiol 301:F813-22 (2011). IHC-Fr ; Mouse . Read more (PubMed: 21775483) »
