Anti-53BP1 antibody (ab21083)
- Product nameAnti-53BP1 antibodySee all 53BP1 primary antibodies ...
- DescriptionRabbit polyclonal to 53BP1
- SpecificityRecognises 53BP1
- Tested applicationsWB, ICC/IF, IHC-P more details
- Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to the carboxy-terminus of h53BP1, conjugated to a carrier protein.
- Positive controlHeLa whole cell lysate
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: PBS, pH 7.2
- Concentration information loading...
- PurityImmunogen affinity purified
- Clonality Polyclonal
- Research Areas
Our Abpromise guarantee covers the use of ab21083 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: Use at an assay dependent dilution. Detects a band of approximately 250 kDa (predicted molecular weight: 220 kDa).|
|ICC/IF||ICC/IF: 1/200. Used at a dilution of 1/200 for 30 min incubation (see Abreview submitted by Kirk McManus).|
|IHC-P||IHC-P: Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
- FunctionMay have a role in checkpoint signaling during mitosis. Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage.
- Involvement in diseaseNote=A chromosomal aberration involving TP53BP1 is found in a form of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;15)(q33;q22) with PDGFRB creating a TP53BP1-PDGFRB fusion protein.
- Sequence similaritiesContains 2 BRCT domains.
modificationsAsymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding.
Phosphorylated at basal level in the absence of DNA damage. Hyper-phosphorylated in an ATM-dependent manner in response to DNA damage induced by ionizing radiation. Hyper-phosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation.
- Cellular localizationNucleus. Chromosome > centromere > kinetochore. Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. Methylation of histone H4 at 'Lys-20' is required for efficient localization to double strand breaks.
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Anti-53BP1 antibody images
Predicted band size : 220 kDa
Western blot analysis of 53BP1 expression in HeLa whole cell lysate.
ab21083 (2µg/ml) staining 53BP1 in human Brain: Cerebellum using an automated system (DAKO Autostainer Plus). Using this protocol there is ubiquitous nuclear staining throughout.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
References for Anti-53BP1 antibody (ab21083)
This product has been referenced in:
- Glass M & Everett RD Components of promyelocytic leukemia nuclear bodies (ND10) act cooperatively to repress herpesvirus infection. J Virol 87:2174-85 (2013). Read more (PubMed: 23221561) »
- Popuri V et al. RECQL5 plays co-operative and complementary roles with WRN syndrome helicase. Nucleic Acids Res : (2012). ICC/IF . Read more (PubMed: 23180761) »