Anti-53BP1 antibody (ab21083)

Overview

• Product nameAnti-53BP1 antibodySee all 53BP1 primary antibodies ...
• Description
  Rabbit polyclonal to 53BP1
• SpecificityRecognises 53BP1
• Tested applicationsWB, ICC/IF, IHC-P more details
• Species reactivity
  Reacts with: Mouse, Human
  
• Immunogen

  Synthetic peptide corresponding to the carboxy-terminus of h53BP1, conjugated to a carrier protein.

• Positive controlHeLa whole cell lysate

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: PBS, pH 7.2
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Oncoproteins/suppressors
  • Tumor suppressors
  • p53 pathway

  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Damage & Repair
  • DNA Damage Response
  • p53

  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Damage & Repair
  • DNA Damage Response
  • BRCT Domain Proteins

Applications

Target

• FunctionMay have a role in checkpoint signaling during mitosis. Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage.
• Involvement in diseaseNote=A chromosomal aberration involving TP53BP1 is found in a form of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;15)(q33;q22) with PDGFRB creating a TP53BP1-PDGFRB fusion protein.
• Sequence similaritiesContains 2 BRCT domains.
• Post-translational
  modificationsAsymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding.
  Phosphorylated at basal level in the absence of DNA damage. Hyper-phosphorylated in an ATM-dependent manner in response to DNA damage induced by ionizing radiation. Hyper-phosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation.
• Cellular localizationNucleus. Chromosome > centromere > kinetochore. Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. Methylation of histone H4 at 'Lys-20' is required for efficient localization to double strand breaks.

Target information above from: UniProt accession Q12888 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 7158 Human
  • Entrez Gene: 27223 Mouse
  • Omim: 605230 Human
  • SwissProt: Q12888 Human
  • SwissProt: P70399 Mouse
  • Unigene: 440968 Human
  • Unigene: 383499 Mouse
  • Unigene: 481841 Mouse

• Alternative names
  53 BP1 antibody53BP1 antibodyFLJ41424 antibody

  MGC138366 antibodyp202 antibodyp53 binding protein 1 antibodyp53 BP1 antibodyp53-binding protein 1 antibodyp53BP1 antibodyTP53 BP1 antibodyTP53B_HUMAN antibodyTP53BP1 antibodyTRP53 BP1 antibodyTumor protein 53 binding protein 1 antibodyTumor protein p53 binding protein 1 antibodyTumor suppressor p53 binding protein 1 antibodyTumor suppressor p53-binding protein 1 antibody

  see all

Anti-53BP1 antibody images

• 

  Western blot - Anti-53BP1 antibody (ab21083)
  
  
  Predicted band size : 220 kDa
  
  
  Western blot analysis of 53BP1 expression in HeLa whole cell lysate.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-53BP1 antibody(ab21083)
  ab21083 (2µg/ml) staining 53BP1 in human Brain: Cerebellum using an automated system (DAKO Autostainer Plus). Using this protocol there is ubiquitous nuclear staining throughout.
  Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.