Overview
- Product nameAnti-CD105 antibodySee all CD105 primary antibodies ...
- DescriptionRabbit polyclonal to CD105
- Tested applicationsSandwich ELISA, ELISA, WB, Flow Cyt more details
- Species reactivityReacts with: Human
- Immunogen
Highly pure recombinant human soluble CD105 (aas 26-586) bearing C-terminal His 6 tag.
Properties
- FormLiquid
- Storage instructionsStore at +4°C. Aliquot and store at -20°C or -80°C when reconstituted. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: PBS, pH 7.4 -
Concentration information loading... - PurityProtein A purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab21224 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Sandwich ELISA | sELISA: Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [MEM-226] to CD105 (ab2529). For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [MEM-226] to CD105 (ab2529) as Capture. |
| ELISA | ELISA: Use a concentration of 1 - 15 µg/ml. |
| WB | WB: Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 71 kDa. |
| Flow Cyt | Flow Cyt: Use at an assay dependent concentration. |
Target
- FunctionMajor glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors.
- Tissue specificityEndoglin is restricted to endothelial cells in all tissues except bone marrow.
- Involvement in diseaseDefects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity.
- Cellular localizationMembrane.
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Database links
- Entrez Gene: 2022 Human
- Omim: 131195 Human
- SwissProt: P17813 Human
- Unigene: 76753 Human
Target information above from: UniProt accession
P17813
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- CD 105 antibodyCD105 antibodyCD105 antigen antibody
- EGLN_HUMAN antibodyEND antibodyEndoglin antibodyENG antibodyFLJ41744 antibodyHHT1 antibodyORW antibodyORW1 antibodyOsler Rendu Weber syndrome 1 antibodyRP11 228B15.2 antibodySN6 antibody
see all
Anti-CD105 antibody images
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Sandwich ELISA - CD105 antibody (ab21224)Standard Curve for CD105 (Analyte: CD105 protein (ab54338)); dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [MEM-226] to CD105 (ab2529) at 5ug/ml and Detector Antibody Rabbit polyclonal to CD105 (ab21224) at 0.5ug/ml. -
Flow Cytometry - Anti-CD105 antibody (ab21224)Image courtesy of Dr A Jackson by Abreview.Flow Cytometry analysis of CD105 in human endothelial precursor cells using ab21224 undiluted. The secondary used was an undiluted Allophycocyanin conjugated goat anti-rabbit polyclonal.Red line= ab21224Black line= isotype control
Protocols
References for Anti-CD105 antibody (ab21224)
ab21224 has not yet been referenced specifically in any publications.
