Anti-ASF1A antibody (ab22231)

Overview

• Product nameAnti-ASF1A antibodySee all ASF1A primary antibodies ...
• Description
  Mouse polyclonal to ASF1A
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Chicken, Cow
• Immunogen

  Fusion protein: RFHINWEDNTEKLEDAESSN, corresponding to amino acids 148/167 of Human ASF1a

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• General notesProduced from outbred CD1 mice

  
  

  This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
• Storage bufferConstituents: 50% Glycerol
• PurityWhole antiserum
• Primary antibody notes This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Damage & Repair
  • DNA Damage Response
  • Other

  • Epigenetics and Nuclear Signaling
  • Chromatin Remodeling
  • Histone chaperones

  • Epigenetics and Nuclear Signaling
  • Chromatin Remodeling
  • Polycomb Silencing
  • Other

Applications

Target

• FunctionHistone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.
• Tissue specificityUbiquitously expressed.
• Sequence similaritiesBelongs to the ASF1 family.
• Post-translational
  modificationsPhosphorylated by TLK1 and TLK2. Highly phosphorylated in S-phase and at lower levels in M-phase.
• Cellular localizationNucleus.

Target information above from: UniProt accession Q9Y294 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 723780 Chicken
  • Entrez Gene: 618099 Cow
  • Entrez Gene: 25842 Human
  • Entrez Gene: 66403 Mouse
  • Omim: 609189 Human
  • SwissProt: Q3C1E9 Chicken
  • SwissProt: Q2KIG1 Cow
  • SwissProt: Q9Y294 Human

  • SwissProt: Q9CQE6 Mouse
  • Unigene: 292316 Human
  • Unigene: 272989 Mouse

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• Alternative names
  Anti silencing function 1A antibodyAnti-silencing function protein 1 homolog A antibodyASF1 anti silencing function 1 homolog A (S. cerevisiae) antibody

  ASF1 anti silencing function 1 homolog A antibodyasf1a antibodyASF1A_HUMAN antibodyCCG1 interacting factor A antibodyCCG1-interacting factor A antibodyCGI 98 antibodyCGI 98 protein antibodyCIA antibodyhAsf1 antibodyhAsf1a antibodyhCIA antibodyHistone chaperone ASF1A antibodyHSPC146 antibodyHypothetical protein DKFZp564E2182 antibodyHypothetical protein FLJ22085 antibody

  see all

Anti-ASF1A antibody images

• 

  Western blot - ASF1a antibody (ab22231)
  All lanes : Anti-ASF1A antibody (ab22231) at 1/1000 dilution
  
  Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a negative control fusion protein with an irrelevant antigen at 20 ug
  Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the antigen fusion protein at 20 ug
  
  Secondary
  Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution
  
  Predicted band size : 23 kDa