Anti-acetyl Lysine antibody [1C6] (ab22550)

Overview

• Product nameAnti-acetyl Lysine antibody [1C6]See all acetyl Lysine primary antibodies ...
• Description
  Mouse monoclonal [1C6] to acetyl Lysine
• Specificityab22550 recognises proteins with acetylated lysine.
• Tested applicationsICC/IF, WB more details
• Species reactivity
  Reacts with: Mouse, Human
  
• Immunogen

  Synthetic peptide: sequence surrounding the acetylated lysine 9 of histone H3

• Positive controlHeLa cell lysate. MCF7 cell line.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.05% Sodium Azide
  Constituents: PBS, 1mg/ml BSA
• Concentration information loading...
• PurityProtein G purified
• Clonality Monoclonal
• Clone number1C6
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • Histones
  • H2A
  • Acetylated

  • Epigenetics and Nuclear Signaling
  • Histones
  • H3
  • Acetylated

  • Epigenetics and Nuclear Signaling
  • Histones
  • H4
  • Acetylated

  • Epigenetics and Nuclear Signaling
  • Histones
  • H2B
  • Acetylated

Applications

Target

• RelevanceIn the nucleus, DNA is tightly packed into nucleosomes generating an environment which is highly repressive towards DNA processes such as transcription. Acetylation of lysine residues within proteins has emerged as an important mechanism used by cells to overcome this repression. The acetylation of non-histone proteins such as transcription factors, as well as histones appears to be involved in this process. Acetylation may result in structural transitions as well as specific signaling within discrete chromatin domains. The role of acetylation in intracellular signaling has been inferred from the binding of acetylated peptides by the conserved bromodomain. Furthermore, recent findings suggest that bromodomain/acetylated-lysine recognition can serve as a regulatory mechanism in protein-protein interactions in numerous cellular processes such as chromatin remodeling and transcriptional activation. The reversible lysine acetylation of histones and non-histone proteins plays a vital role in the regulation of many cellular processes including chromatin dynamics and transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication, DNA repair, nuclear import, and neuronal repression. More than 20 acetyltransferases and 18 deacetylases have been identified so far, but the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. Over 40 transcription factors and 30 other nuclear, cytoplasmic, bacterial, and viral proteins have been shown to be acetylated in vivo.

• Alternative names
  acetyl Lysine antibodyLysine antibody

Anti-acetyl Lysine antibody [1C6] images

• 

  Immunocytochemistry/ Immunofluorescence - Anti-acetyl Lysine antibody [1C6] (ab22550)
  ICC/IF image of ab22550 stained MCF7 cells. The cells were 4% formaldehye fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22550, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.