Anti-EDD antibody - C-terminal (ab226792)
Key features and details
- Rabbit polyclonal to EDD - C-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-EDD antibody - C-terminal
See all EDD primary antibodies -
Description
Rabbit polyclonal to EDD - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow -
Immunogen
Recombinant fragment within Human EDD (C terminal). The exact sequence is proprietary.
Database link: O95071 -
Positive control
- WB: U-87 MG whole cell lysate and nuclear extract. ICC/IF: U-87 MG cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab226792 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 309 kDa.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 309 kDa. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific amino-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation (By similarity). May be involved in maturation and/or transcriptional regulation of mRNA. May play a role in control of cell cycle progression. May have tumor suppressor function. Regulates DNA topoisomerase II binding protein (TopBP1) in the DNA damage response. Plays an essential role in extraembryonic development. -
Tissue specificity
Widely expressed. Most abundant in testis and expressed at high levels in brain, pituitary and kidney. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
Contains 1 PABC domain.
Contains 1 UBR-type zinc finger. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 515582 Cow
- Entrez Gene: 51366 Human
- Entrez Gene: 70790 Mouse
- Omim: 608413 Human
- SwissProt: O95071 Human
- SwissProt: Q80TP3 Mouse
- Unigene: 492445 Human
- Unigene: 476840 Mouse
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Alternative names
- 4432411E13Rik antibody
- AW549941 antibody
- C77315 antibody
see all
Images
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All lanes : Anti-EDD antibody - C-terminal (ab226792) at 1/500 dilution
Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 2 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) nuclear extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 309 kDa -
4% paraformaldehyde-fixed, U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cells stained for EDD (green) using ab226792 at 1/500 dilution in ICC/IF.
Alpha-Tubulin is detected with an anti-alpha-Tubulin antibody at 1/1000 dilution (red).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab226792 has not yet been referenced specifically in any publications.