Human Pro-Collagen I alpha 1 ELISA Kit, Fluorescent (ab229389)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 3.7 pg/ml
- Range: 3.91 pg/ml - 16000 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
- Detection method: Fluorescent
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Pro-Collagen I alpha 1 ELISA Kit, Fluorescent
See all Procollagen Type 1 kits -
Detection method
Fluorescent -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 1.8% Inter-assay Sample n Mean SD CV% Serum 3 3% -
Sample type
Cell culture supernatant, Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
3.7 pg/ml -
Range
3.91 pg/ml - 16000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 93 91% - 94% Cell culture media 99 97% - 101% Hep Plasma 101 94% - 107% EDTA Plasma 108 105% - 114% Cit plasma 106 102% - 110% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Cow -
Product overview
Pro-Collagen I alpha 1 in vitro CatchPoint SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Pro-Collagen I alpha 1 protein in human serum, plasma, cell culture supernatants, and cell and tissue extract samples.
This CatchPoint SimpleStep ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
If using a Molecular Devices’ plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org.
The CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.
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Notes
Type I collagen is the most abundant structural protein of connective tissues such as skin, bone and tendon. It is synthesized as a pro-collagen molecule that is characterized by a 300 nm triple helical domain flanked by globular N- and C-terminal propeptides. Specifically, human Pro-Collagen I alpha 1 consists of a signal peptide (amino acids (aa) 1-22), a propeptide (aa 23-161), the mature chain (aa 162-1218), and another propeptide (aa 1219 – 1464). The non-helical propeptides are removed by procollagen N- and C-proteinase activities so that the mature triple helices can self-assemble into collagen fibrils that provide tensile strength to tissues.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 1 x 96 tests 100X Stoplight Red Substrate 1 x 120µl 1 x 120µl 10X Human Pro-Collagen I alpha 1 Capture Antibody 1 x 600µl 1 x 600µl 10X Human Pro-Collagen I alpha 1 Detector Antibody 1 x 600µl 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 1 x 20ml 500X Hydrogen Peroxide (H2O2, 3%) 1 x 50µl 1 x 50µl 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml 1 x 10ml Antibody Diluent CPI2 1 x 6ml 1 x 6ml Human Pro-Collagen I alpha 1 Lyophilized Recombinant Protein 2 vials 2 vials Plate Seals 1 unit 1 unit Sample Diluent NS (ab193972) 1 x 50ml 1 x 50ml SimpleStep Pre-Coated Black 96-Well Microplate 1 unit 1 unit Stoplight Red Substrate Buffer 1 x 12ml 1 x 12ml -
Research areas
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Function
Type I collagen is a member of group I collagen (fibrillar forming collagen). -
Tissue specificity
Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite. -
Involvement in disease
Caffey disease
Ehlers-Danlos syndrome, classic type
Ehlers-Danlos syndrome 7A
Osteogenesis imperfecta 1
Osteogenesis imperfecta 2
Osteogenesis imperfecta 3
Osteogenesis imperfecta 4
Osteoporosis
A chromosomal aberration involving COL1A1 is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF. -
Sequence similarities
Belongs to the fibrillar collagen family.
Contains 1 fibrillar collagen NC1 domain.
Contains 1 VWFC domain. -
Domain
The C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function. -
Post-translational
modificationsProline residues at the third position of the tripeptide repeating unit (G-X-P) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-P-X) are hydroxylated in some of the chains.
O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group. -
Cellular localization
Secreted, extracellular space, extracellular matrix. - Information by UniProt
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Alternative names
- Alpha 1 type I collagen
- Alpha 2 type I collagen
- Alpha-1 type I collagen
see all -
Database links
- Entrez Gene: 1278 Human
- Entrez Gene: 1277 Human
- SwissProt: P08123 Human
- SwissProt: P02452 Human
- Unigene: 172928 Human
- Unigene: 681002 Human
Images
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of Pro-Collagen I alpha 1 were measured in duplicates, interpolated from the Pro-Collagen I alpha 1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1%, plasma (citrate) 1%, plasma (EDTA) 1%, and plasma (heparin) 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Pro-Collagen I alpha 1 concentration was determined to be 142.1 ng/mL in serum, 135.9 ng/mL in plasma (citrate), 112.1 ng/mL in plasma (EDTA) and 102.1 ng/mL in plasma (heparin).
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The concentrations of Pro Collagen I alpha 1 were measured in duplicate and interpolated from the Pro Collagen I alpha 1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Pro-Collagen I alpha 1 concentration was determined to be 1.62 ng/mL in IMR-90 extract.
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Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Pro-Collagen I alpha 1 concentration was determined to be 197.3 ng/mL with a range of 113.0 – 417 ng/mL.
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To learn more about the advantages of recombinant antibodies see here.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab229389 has been referenced in 2 publications.
- Qin D et al. Gremlin mediates the TGF-ß-induced induction of profibrogenic genes in human retinal pigment epithelial cells. Exp Ther Med 19:2353-2359 (2020). PubMed: 32104303
- Fang CH et al. Biomimetic Synthesis of Nanocrystalline Hydroxyapatite Composites: Therapeutic Potential and Effects on Bone Regeneration. Int J Mol Sci 20:N/A (2019). PubMed: 31795225