Overview
- Product nameAnti-15 Lipoxygenase 2 antibodySee all 15 Lipoxygenase 2 primary antibodies ...
- DescriptionRabbit polyclonal to 15 Lipoxygenase 2
- SpecificityThis antibody recognises 15 Lipoxygenase 2. It does not recognise 15 lipoxygenase 1 and 12 lipoxygenase 1.
- Tested applicationsIHC-Fr, ICC/IF, WB, ICC more details
- Species reactivityReacts with: Human
Does not react with
Rabbit, Pig - Immunogen
Synthetic peptide: CLDEKTVEDLELNIKYSTA, corresponding to amino acids 163-181 of Human 15 Lipoxygenase 2.
- Positive controlHuman prostate homogenate.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, 0.1% BSA, Tris buffered saline, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesThis antibody was affinity purified.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab23691 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| IHC-Fr | |
| ICC/IF | |
| WB | |
| ICC |
WB: Use at a concentration of 3 µg/ml or 1:200. Predicted molecular weight: 76 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
- FunctionConverts arachidonic acid exclusively to 15S-hydroperoxyeicosatetraenoic acid, while linoleic acid is less well metabolized.
- Tissue specificityExpressed in hair, prostate, lung and cornea.
- PathwayLipid metabolism; leukotriene D4 biosynthesis.
- Sequence similaritiesBelongs to the lipoxygenase family.
Contains 1 lipoxygenase domain.
Contains 1 PLAT domain. - Cellular localizationCytoplasm.
-
Database links
- Entrez Gene: 247 Human
- Omim: 603697 Human
- SwissProt: O15296 Human
- Unigene: 111256 Human
Target information above from: UniProt accession
O15296
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
-
Alternative names
- 15 LO 2 antibody15 LOX 2 antibody15-lipoxygenase 2 antibody
- 15-LOX-2 antibody15-LOX-B antibodyALOX15B antibodyArachidonate 15 lipoxygenase second type antibodyArachidonate 15 lipoxygenase type II antibodyArachidonate 15-lipoxygenase B antibodyArachidonate 15-lipoxygenase type II antibodyLX15B_HUMAN antibody
see all
Anti-15 Lipoxygenase 2 antibody images
-
Western blot - 15 Lipoxygenase 2 antibody (ab23691)All lanes : Anti-15 Lipoxygenase 2 antibody (ab23691) at 4 µg/ml
Lane 1 : Rabbit reticulocyte lysate (30 µg).
Lane 2 : Human prostate homogenate (30 µg).
Predicted band size : 76 kDa
Observed band size : 76 kDa -
Immunohistochemistry (Frozen sections) - 15 Lipoxygenase 2 antibody (ab23691)This image is courtesy of an anonymous Abreviewab23691 staining 15 Lipoxygenase 2 in human artery tissue sections by IHC-Fr (frozen sections). Tissue samples were fixed with acetone and blocked with 0.5% BSA for 10 minutes. Samples were incubated with primary antibody 1/100 24 hours. A biotin-conjugated horse anti Rabbit IgG (H+L) polyclonal (7 µg/ml) was used as secondary antibody. -
Immunocytochemistry/ Immunofluorescence - Anti-15 Lipoxygenase 2 antibody (ab23691)This image is courtesy of an anonymous AbreviewImmunofluorescence analysis of murine embryonic fibroblasts, staining 15 Lipoxygenase 2 with ab23691.
Cells were fixed with paraformaldehyde, permeabilized with 0.2 % Triton-X-100 and blocked with 2% BSA for 1 hour at 20°C. Samples were incubated with primary antibody (1/100 in 1% BSA in TBST) for 16 hours at 4°C. An AlexaFluor®488-conjugated chicken anti-rabbit polyclonal IgG (1/2000) was used as the secondary antibody.
References for Anti-15 Lipoxygenase 2 antibody (ab23691)
ab23691 has not yet been referenced specifically in any publications.
