Overview
- Product nameAnti-AGE antibody
- DescriptionRabbit polyclonal to AGE
- Specificityab23722 reacts with Advanced Glycation End Products (AGE), Cross-reacts with BSA and HSA < 1%
- Tested applicationsWB, ICC/IF, ELISA, IHC-Fr, IHC-P more details
- Immunogen
Advanced Glycation End Products (BSA-AGE and HSA-AGE)
- Positive controlHuman lens, arteriosclerotic plaques
- General notes
ab23722 is suitable for the detection of different AGE products in tissues, tissue extracts and body fluids.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.05% Sodium Azide
Constituents: 0.85% Sodium chloride, 0.05M PBS, pH 7.15 -
Concentration information loading... - PurityProtein A purified
- Primary antibody notes ab23722 is suitable for the detection of different AGE products in tissues, tissue extracts and body fluids.
- Clonality Polyclonal
- IsotypeIgG
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Research Areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipoprotein metabolism
Applications
Our Abpromise guarantee covers the use of ab23722 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use at an assay dependent concentration. Can be blocked with AGE-BSA protein (ab129535). |
| ICC/IF | ICC/IF: Use a concentration of 5 µg/ml. |
| ELISA | ELISA: Use a concentration of 1 µg/ml. |
| IHC-Fr | IHC-Fr: Use at an assay dependent concentration. |
| IHC-P | IHC-P: 1/10000. PubMed: 19223295 |
Target
- RelevanceThe non enzymatic reaction of reducing carbohydrates with lysine side chains and N terminal amino groups of macromolecules (amino acids, proteins, phospholipids and nucleic acids) is called the Maillard reaction or glycation. The latter products of this process, termed advanced glycation end products (AGEs), adversely affect the functional properties of proteins, lipids and DNA. In long lived tissue proteins, these chemical modifications accumulate with age and may contribute to the pathophysiology of ageing and long term complications of diabetes, atherosclerosis and renal failure.
- Cellular localizationCell Membrane and Secreted
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Alternative names
- Advanced glycation end products antibodyAGEs antibody
Anti-AGE antibody images
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Western blot - Anti-AGE antibody (ab23722)This image is courtesy of an Abreview submitted by Josephine BöhmeAll lanes : Anti-AGE antibody (ab23722) at 1/1000 dilution
Lane 1 : Mouse Brain Lysate(Brain and Hippocampus) at 25 µg
Lane 2 : Glycated BSA at 15 µg
Secondary
HRP-conjugated Goat anti-rabbit IgG at 1/800 dilution
Performed under reducing conditions.This image is courtesy of an Abreview submitted by Josephine Böhme
12% SDS-PAGE gel run using denaturing conditions. Membrane blocked with 5% BSA for 30 minutes at room temperature. Primary antibody diluted in 5% BSA in TBS-T and incubated for 20 hours in a fridge
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-AGE antibody(ab23722)ab23722 (1µg/ml) staining AGE in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of both cell membrane and cytoplasm.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required. -
Immunocytochemistry/ Immunofluorescence-AGE antibody(ab23722)ICC/IF image of ab23722 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab23722, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-AGE antibody (ab23722)
This product has been referenced in:
- Medford HM et al. Chronic ingestion of a Western diet increases O-linked-ß-N-acetylglucosamine (O-GlcNAc) protein modification in the rat heart. Life Sci 90:883-8 (2012). WB ; Rat . Read more (PubMed: 22575823) »
- Raposeiras-Roubín S et al. Soluble receptor of advanced glycation end products levels are related to ischaemic aetiology and extent of coronary disease in chronic heart failure patients, independent of advanced glycation end products levels: New Roles for Soluble RAGE. Eur J Heart Fail 12:1092-100 (2010). ELISA ; Human . Read more (PubMed: 20685687) »
