Overview
- Product nameAnti-LXR alpha + LXR beta antibodySee all LXR alpha + LXR beta primary antibodies ...
- DescriptionGoat polyclonal to LXR alpha + LXR beta
- Tested applicationsWB more details
- Species reactivityReacts with: Mouse, Rabbit, Human
Predicted to work with: Rat, Chicken, Cow, Pig, Xenopus laevis, Zebrafish - Immunogen
Peptide with sequence (C)RLQDKKLPPLLSEI, from the C-terminus of the human protein sequences according to NP_005684; NP_009052.
- Positive controlHuman Spleen lysate.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline. pH 7.3 -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab24362 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa). |
Target
- RelevanceLXR alpha and LXR beta are orphan receptors. Interaction with RXR shifts RXR from its role as a silent DNA binding partner to an active ligand binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4 type response elements characterized by direct repeats of two similar hexanuclotide half sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis. LXR alpha: Visceral organs specific expression. Strong expression was found in liver, kidney and intestine followed by spleen and to a lesser extent the adrenals. LXR beta: Ubiquitously expressed.
- Cellular localizationNuclear
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Database links
- Entrez Gene: 395221 Chicken
- Entrez Gene: 507176 Cow
- Entrez Gene: 10062 Human
- Entrez Gene: 7376 Human
- Entrez Gene: 22259 Mouse
- Entrez Gene: 397553 Pig
- Entrez Gene: 58852 Rat
- Omim: 602423 Human
- SwissProt: Q5E9B6 Cow
- SwissProt: Q13133 Human
- SwissProt: P55055 Human
- SwissProt: Q9Z0Y9 Mouse
- SwissProt: Q91X41 Mouse
- SwissProt: Q62685 Rat
- SwissProt: Q5I035 Rat
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Alternative names
- Liver X receptor alpha antibodyLiver X receptor alpha antibodyLiver X receptor beta antibody
- LXR a antibodyLXR antibodyLXR b antibodyLXRa antibodyLXRalpha antibodyLXRb antibodyLXRbeta antibodyNER antibodyNERI antibodyNR1H2 antibodyNR1H3 antibodyNuclear orphan receptor LXR alpha antibodyNuclear orphan receptor LXR beta antibodyNuclear receptor NER antibodyNuclear receptor subfamily 1 group H member 2 antibodyNuclear receptor subfamily 1 group H member 3 antibodyOxysterols receptor LXR alpha antibodyOxysterols receptor LXR beta antibodyRIP15 antibodyRLD1 antibodyUbiquitously expressed nuclear receptor antibodyUNR antibody
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Anti-LXR alpha + LXR beta antibody images
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Western blot - LXR alpha + LXR beta antibody (ab24362)Anti-LXR alpha + LXR beta antibody (ab24362) at 1 µg/ml + Human spleen lysate(in RIPA buffer) at 35 µg
developed using the ECL technique
Predicted band size : 51 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
Additional bands at : 38 kDa,75 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 1 hour
References for Anti-LXR alpha + LXR beta antibody (ab24362)
This product has been referenced in:
- Donmez G et al. SIRT1 Suppresses beta-Amyloid Production by Activating the alpha-Secretase Gene ADAM10. Cell 142:320-332 (2010). WB ; Mouse . Read more (PubMed: 20655472) »
