Anti-NSE antibody [1C1] (HRP) - Neuronal Marker (ab24472)

Overview

• Product nameAnti-NSE antibody [1C1] (HRP) - Neuronal MarkerSee all NSE primary antibodies ...
• Description
  Mouse monoclonal [1C1] to NSE - Neuronal Marker (HRP)
• ConjugationHRP
• SpecificityReacts with 45 kD band corresponding to NSE in immunoblotting of human brain extracts
• Tested applicationsELISA, Sandwich ELISA, WB more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Purified Neuron Specific Enolase from human brain.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
• Storage bufferpH: 7.40
  Constituents: PBS, 50% Glycerol, 1% BSA
• Concentration information loading...
• PurityProtein G purified
• Clonality Monoclonal
• Clone number1C1
• MyelomaSp2/0
• IsotypeIgG2a
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Carbohydrate metabolism

  • Developmental Biology
  • Lineage specification
  • Ectoderm

  • Stem Cells
  • Lineage Markers
  • Ectoderm

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Metabolism of carbohydrates

  • Cancer
  • Tumor biomarkers
  • Enzymes
  • Neuron Specific Enolase

  • Tags & Cell Markers
  • Cell Type Markers
  • Neuroscience Markers
  • Glial

  • Neuroscience
  • Cell Type Marker
  • Neuron marker
  • Soma marker

Applications

Target

• FunctionHas neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
• Tissue specificityThe alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
• PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
• Sequence similaritiesBelongs to the enolase family.
• Developmental stageDuring ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
• Cellular localizationCytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.

Target information above from: UniProt accession P09104 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 2026 Human
  • Omim: 131360 Human
  • SwissProt: P09104 Human
  • Unigene: 511915 Human

• Alternative names
  2 phospho D glycerate hydrolyase antibody2-phospho-D-glycerate hydro-lyase antibodyEno 2 antibody

  Eno2 antibodyENOG antibodyENOG_HUMAN antibodyEnolase 2 antibodyEnolase 2 gamma neuronal antibodyEnolase2 antibodyGamma enolase antibodyGamma-enolase antibodyNeural enolase antibodyNeuron specific enolase antibodyNeuron specific gamma enolase antibodyNeuron-specific enolase antibodyNeurone specific enolase antibodyNSE antibody

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Anti-NSE antibody [1C1] (HRP) - Neuronal Marker images

• 

  Western blot - Anti-NSE antibody [1C1] (HRP) - Neuronal Marker (ab24472)
  All lanes : Anti-NSE antibody [1C1] (HRP) - Neuronal Marker (ab24472) at 1/1000 dilution
  
  Lane 2 : NSE protein (Human) (ab78797) at 5 µg
  Lane 3 : NSE protein (Human) (ab78797) at 0.005 µg
  Lane 4 : Whole tissue lysate prepared from human brain at 15 µg
  
  developed using the ECL technique
  
  Observed band size : 45 kDa (why is the actual band size different from the predicted?)
  
  
  Exposure time : 1 minute

  Image courtesy of Dr Rachel Barker by Abreview.

  See Abreview