You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Anti-6X His tag® antibody (Biotin) (ab27025)

Abreviews (3)Q&A (2)Specific References (2)

Overview

  • Product nameAnti-6X His tag® antibody (Biotin)See all 6X His tag® primary antibodies ...
  • Description
    Rabbit polyclonal to 6X His tag® (Biotin)
  • ConjugationBiotin
  • SpecificityThis antibody recognises 6x His tag.
  • Tested applicationsFlow Cyt, WB, ELISA more details
  • Immunogen

    HHHHHH conjugated with KLH.

  • General notesHIS-TAG® is a trademark of EMD Biosciences, Inc.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.2% BSA, PBS, pH 7.2
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was purified using the peptide immobilized on a solid support.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab27025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Flow Cyt
WB
ELISA
  • Application notesELISA: 1/2000 - 1/10000.
    WB: 1/1000 - 1/5000.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • RelevanceThe H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
    • Cellular localizationDepends upon the localization of the parent protein tagged with hexahistidine.
    • Alternative names
        6 His epitope tag antibodyHexa His tag antibodyHHHHHH epitope tag antibody
        HHHHHH tag antibodyHis tag antibody
      see all

    References for Anti-6X His tag® antibody (Biotin) (ab27025)

    This product has been referenced in:
    • Spång HC  et al. Heterodimeric Barnase-Barstar Vaccine Molecules: Influence of One versus Two Targeting Units Specific for Antigen Presenting Cells. PLoS One 7:e45393 (2012). WB . Read more (PubMed: 23028981) »
    • Lee HK  et al. In vivo requirement for Atg5 in antigen presentation by dendritic cells. Immunity 32:227-39 (2010). Read more (PubMed: 20171125) »

    See all 2 Publications for this product

    Publishing research using ab27025 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab27025

    Filter by Application

    Filter by Species

    Filter by Ratings

    Displaying 1 - 3 of 3 results for Abreviews

    10
    Votes: Highest First

    Western blot abreview for Anti-6X His tag® antibody (Biotin)

     Good
    Abreviews
    Application Western blot
    Sample Escherichia coli Purified protein (recombinant human fibronectin domain)
    Loading amount 0.1 µg
    Specification recombinant human fibronectin domain
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    Username

    Verified customer

    Submitted Sep 04 2008

    Flow Cytometry abreview for Anti-6X His tag® antibody (Biotin)

     Excellent
    Abreviews
    Application Flow Cytometry
    Sample Saccharomyces cerevisiae Cell (yeast expressing a his tagged scFv)
    Specification yeast expressing a his tagged scFv
    Preparation Cell harvesting/tissue preparation method: yeast washed once in PBS
    Sample buffer: PBS
    Fixation none
    Permeabilization No
    Gating Strategy sample was gated to include all yeast cells
    Read More
    abreview image
    Username

    Verified customer

    Submitted Jun 24 2008

    Western blot abreview for Anti-6X His tag® antibody (Biotin)

     Excellent
    Abreviews
    Application Western blot
    Sample Human Recombinant protein (Samples pre and post enzymatic his tag cleavage)
    Loading amount 4 µg
    Specification Samples pre and post enzymatic his tag cleavage
    Treatment His tag purified supernatant followed by a reaction to cleave the his tag
    Gel Running Conditions Non-reduced Denaturing (12%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More
    abreview image
    Username

    Verified customer

    Submitted Jun 04 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"