Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043)
- Product nameAnti-58K Golgi protein antibody [58K-9] - Golgi MarkerSee all 58K Golgi protein primary antibodies ...
- DescriptionMouse monoclonal [58K-9] to 58K Golgi protein - Golgi Marker
- Tested applicationsWB, Functional Studies, Flow Cyt, ICC/IF more details
- Species reactivityReacts with: Rat, Hamster, Cow, Dog, Human, Monkey, African Green Monkey
Predicted to work with: Pig
Full length native protein (purified) (Rat liver).
- Positive controlRat Liver. For indirect immunofluorescence: cultured Chinese hamster ovary (CHO) cells For immunoblotting (colorimetric): whole rat liver extract Antigen M.W.: 58 kDa
- General notesWe can conjugate this antibody to FITC for you (please see ab150248 for details).
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Store in the dark. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.5
- Concentration information loading...
- PurityIgG fraction
- Clonality Monoclonal
- Clone number58K-9
- Research Areas
Our Abpromise guarantee covers the use of ab27043 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).|
|Functional Studies||FuncS: Use at an assay dependent dilution.|
|Flow Cyt||Flow Cyt: Use 2µg for 106 cells.|
|ICC/IF||ICC/IF: Use a concentration of 5 - 10 µg/ml. Previous batches have worked at the concentration of 1µg/ml. Our current batch appears to work between 5 and 10 µg/ml. Please see the data below for more details.|
- FunctionFolate-dependent enzyme, that displays both transferase and deaminase activity. Serves to channel one-carbon units from formiminoglutamate to the folate pool.
Binds and promotes bundling of vimentin filaments originating from the Golgi.
- PathwayAmino-acid degradation; L-histidine degradation into L-glutamate; L-glutamate from N-formimidoyl-L-glutamate (transferase route): step 1/1.
One-carbon metabolism; tetrahydrofolate interconversion.
- Involvement in diseaseDefects in FTCD are the cause of glutamate formiminotransferase deficiency (FIGLU-URIA) [MIM:229100]; also known as formiminoglutamicaciduria (FIGLU-uria). It is an autosomal recessive disorder. Features of a severe phenotype, include elevated levels of formiminoglutamate (FIGLU) in the urine in response to histidine administration, megaloblastic anemia, and mental retardation. Features of a mild phenotype include high urinary excretion of FIGLU in the absence of histidine administration, mild developmental delay, and no hematological abnormalities.
- Sequence similaritiesIn the C-terminal section; belongs to the cyclodeaminase/cyclohydrolase family.
In the N-terminal section; belongs to the formiminotransferase family.
- Cellular localizationCytoplasm > cytoskeleton > centrosome > centriole. Golgi apparatus. More abundantly located around the mother centriole.
- Formimidoyltetrahydrofolate cyclodeaminase antibodyFormimidoyltransferase cyclodeaminase antibodyFormiminotetrahydrofolate cyclodeaminase antibody
- Formiminotransferase cyclodeaminase antibodyFormiminotransferase cyclodeaminase antibodyFormiminotransferase-cyclodeaminase antibodyFTCD antibodyFTCD antibodyFTCD_HUMAN antibodyGlutamate formiminotransferase antibodyGlutamate formiminotransferase antibodyGlutamate formyltransferase antibodyLCHC 1 antibodyLCHC1 antibodyLCHC1 antibody
Anti-58K Golgi protein antibody [58K-9] - Golgi Marker images
All lanes : Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043) at 1 µg/ml
Lane 1 : Liver (Rat) Tissue Lysate, blocked with 5% BSA
Lane 2 : Liver (Rat) Tissue Lysate, blocked with 3% Milk
Lysates/proteins at 20 µg per lane.
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 58 kDa
Exposure time : 3 minutes
ab27043 (1µg/ml, 5µg/ml and 10µg/ml) staining 58K Golgi protein in SK-N-SH cells (green). Cells were fixed in Methanol, permabilised using 0.5% Triton X100 in PBS and counterstained with DAPI in order to highlight the nucleus (red).
ICC/IF image of ab27043 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab27043, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
Overlay histogram showing HepG2 cells stained with ab27043 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab27043, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
References for Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043)
This product has been referenced in:
- Rodríguez D et al. Functional analysis of sucrase-isomaltase mutations from chronic lymphocytic leukemia patients. Hum Mol Genet N/A:N/A (2013). ICC/IF ; Human . Read more (PubMed: 23418305) »
- Kaniakova M et al. Key Amino Acid Residues within the Third Membrane Domains of NR1 and NR2 Subunits Contribute to the Regulation of the Surface Delivery of N-methyl-D-aspartate Receptors. J Biol Chem 287:26423-34 (2012). ICC/IF . Read more (PubMed: 22711533) »