Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043)

Overview

• Product nameAnti-58K Golgi protein antibody [58K-9] - Golgi MarkerSee all 58K Golgi protein primary antibodies ...
• Description
  Mouse monoclonal [58K-9] to 58K Golgi protein - Golgi Marker
• Tested applicationsWB, Functional Studies, Flow Cyt, ICC/IF more details
• Species reactivity
  Reacts with: Rat, Hamster, Cow, Dog, Human, Monkey, African Green Monkey
  Predicted to work with: Pig
• Immunogen

  Full length native protein (purified) (Rat liver).

• Positive controlRat Liver. For indirect immunofluorescence: cultured Chinese hamster ovary (CHO) cells For immunoblotting (colorimetric): whole rat liver extract Antigen M.W.: 58 kDa
• General notesWe can conjugate this antibody to FITC for you (please see ab150248 for details).

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Store in the dark. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: PBS, pH 7.5
• Concentration information loading...
• PurityIgG fraction
• Clonality Monoclonal
• Clone number58K-9
• IsotypeIgG1
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Amino acid metabolism

  • Metabolism
  • Types of disease
  • Cancer

  • Signal Transduction
  • Metabolism
  • Amino Acids

  • Tags & Cell Markers
  • Subcellular Markers
  • Organelles
  • Golgi

Applications

Target

• FunctionFolate-dependent enzyme, that displays both transferase and deaminase activity. Serves to channel one-carbon units from formiminoglutamate to the folate pool.
  Binds and promotes bundling of vimentin filaments originating from the Golgi.
• PathwayAmino-acid degradation; L-histidine degradation into L-glutamate; L-glutamate from N-formimidoyl-L-glutamate (transferase route): step 1/1.
  One-carbon metabolism; tetrahydrofolate interconversion.
• Involvement in diseaseDefects in FTCD are the cause of glutamate formiminotransferase deficiency (FIGLU-URIA) [MIM:229100]; also known as formiminoglutamicaciduria (FIGLU-uria). It is an autosomal recessive disorder. Features of a severe phenotype, include elevated levels of formiminoglutamate (FIGLU) in the urine in response to histidine administration, megaloblastic anemia, and mental retardation. Features of a mild phenotype include high urinary excretion of FIGLU in the absence of histidine administration, mild developmental delay, and no hematological abnormalities.
• Sequence similaritiesIn the C-terminal section; belongs to the cyclodeaminase/cyclohydrolase family.
  In the N-terminal section; belongs to the formiminotransferase family.
• Cellular localizationCytoplasm > cytoskeleton > centrosome > centriole. Golgi apparatus. More abundantly located around the mother centriole.

Target information above from: UniProt accession O95954 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 10841 Human
  • Entrez Gene: 10841 Human
  • Entrez Gene: 397517 Pig
  • Entrez Gene: 89833 Rat
  • Omim: 606806 Human
  • SwissProt: O95954 Human
  • SwissProt: O95954 Human
  • SwissProt: P53603 Pig

  • SwissProt: O88618 Rat
  • Unigene: 415846 Human
  • Unigene: 20140 Rat

  see all

• Alternative names
  Formimidoyltetrahydrofolate cyclodeaminase antibodyFormimidoyltransferase cyclodeaminase antibodyFormiminotetrahydrofolate cyclodeaminase antibody

  Formiminotransferase cyclodeaminase antibodyFormiminotransferase cyclodeaminase antibodyFormiminotransferase-cyclodeaminase antibodyFTCD antibodyFTCD antibodyFTCD_HUMAN antibodyGlutamate formiminotransferase antibodyGlutamate formiminotransferase antibodyGlutamate formyltransferase antibodyLCHC 1 antibodyLCHC1 antibodyLCHC1 antibody

  see all

Anti-58K Golgi protein antibody [58K-9] - Golgi Marker images

• 

  Western blot - Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043)
  All lanes : Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043) at 1 µg/ml
  
  Lane 1 : Liver (Rat) Tissue Lysate, blocked with 5% BSA
  Lane 2 : Liver (Rat) Tissue Lysate, blocked with 3% Milk
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 58 kDa
  
  
  Exposure time : 3 minutes
• 

  Immunocytochemistry/ Immunofluorescence - Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043)Image courtesy of Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canada
  ab27043 (1µg/ml, 5µg/ml and 10µg/ml) staining 58K Golgi protein in SK-N-SH cells (green). Cells were fixed in Methanol, permabilised using 0.5% Triton X100 in PBS and counterstained with DAPI in order to highlight the nucleus (red).
• 

  Immunocytochemistry/ Immunofluorescence - 58K Golgi protein antibody [58K-9] (ab27043)

  ICC/IF image of ab27043 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab27043, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Image-iT^TM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

• 

  Flow Cytometry-Anti-58K Golgi protein antibody [58K-9] - Golgi Marker(ab27043)
  Overlay histogram showing HepG2 cells stained with ab27043 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab27043, 2µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.