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Anti-Lamin B1 antibody [mAbcam 28129] (ab28129)

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Overview

  • Product nameAnti-Lamin B1 antibody [mAbcam 28129]See all Lamin B1 primary antibodies ...
  • Description
    Mouse monoclonal [mAbcam 28129] to Lamin B1
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Zebrafish
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of Human Lamin B1.

    Read Abcam's proprietary immunogen policy

    (Peptide available as ab16375.)

  • Positive controlThis antibody gave a positive signal in Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab28129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).Can be blocked with Lamin B1 peptide (ab16375).

Target

  • FunctionLamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
  • Involvement in diseaseDefects in LMNB1 are the cause of leukodystrophy demyelinating autosomal dominant adult-onset (ADLD) [MIM:169500]. ADLD is a slowly progressive and fatal demyelinating leukodystrophy, presenting in the fourth or fifth decade of life. Clinically characterized by early autonomic abnormalities, pyramidal and cerebellar dysfunction, and symmetric demyelination of the CNS. It differs from multiple sclerosis and other demyelinating disorders in that neuropathology shows preservation of oligodendroglia in the presence of subtotal demyelination and lack of astrogliosis.
  • Sequence similaritiesBelongs to the intermediate filament family.
  • Post-translational
    modifications    B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
  • Cellular localizationNucleus inner membrane.

    • Target information above from: UniProt accession P20700 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    see all
  • Alternative names
      ADLD antibodylamin B1 antibodyLamin-B1 antibody
      LMB1 antibodyLMN antibodyLMN2 antibodyLMNB 1 antibodyLMNB antibodyLMNB1 antibodyLMNB1_HUMAN antibodyMGC111419 antibodyOTTHUMP00000159218 antibody
    see all

Anti-Lamin B1 antibody [mAbcam 28129] images

  • Western blot - Lamin B1 antibody [mAbcam 28129] (ab28129)
    Western blot - Lamin B1 antibody [mAbcam 28129] (ab28129)
    Anti-Lamin B1 antibody [mAbcam 28129] (ab28129) at 5 µg/ml + Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

    Secondary
    Rabbit polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 70 kDa
    Observed band size : 70 kDa

References for Anti-Lamin B1 antibody [mAbcam 28129] (ab28129)

ab28129 has not yet been referenced specifically in any publications.

Publishing research using ab28129 ? Please let us know so that we can cite the reference in this datasheet

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"