Anti-TATA binding protein TBP antibody - ChIP Grade (ab28175)

Overview

• Product nameAnti-TATA binding protein TBP antibody - ChIP GradeSee all TATA binding protein TBP primary antibodies ...
• Description
  Rabbit polyclonal to TATA binding protein TBP - ChIP Grade
• SpecificityAb28175 recognises TATA binding protein TBP.
• Tested applicationsWB, ChIP more details
• Species reactivity
  Reacts with: Mouse, Human
  
• Immunogen

  Recombinant full length TATA binding protein TBP (Human).

• Positive controlHeLa nuclear extract.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: PBS.
• PurityWhole antiserum
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Isotype/Loading Controls
  • Loading Controls
  • TBP - Nuclear

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Polymerase associated factors
  • Pol II Transcription
  • TAFs

  • Epigenetics and Nuclear Signaling
  • ChIP'ing antibodies
  • ChIP'ing antibodies

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Polymerase associated factors
  • Pol II Transcription
  • TFIIB

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Preinitiation complex

Applications

Target

• FunctionGeneral transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA.
• Tissue specificityWidely expressed, with levels highest in the testis and ovary.
• Involvement in diseaseDefects in TBP are the cause of spinocerebellar ataxia type 17 (SCA17) [MIM:607136]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA17 is an autosomal dominant cerebellar ataxia (ADCA) characterized by widespread cerebral and cerebellar atrophy, dementia and extrapyramidal signs. The molecular defect in SCA17 is the expansion of a CAG repeat in the coding region of TBP. Longer expansions result in earlier onset and more severe clinical manifestations of the disease.
• Sequence similaritiesBelongs to the TBP family.
• Cellular localizationNucleus.

Target information above from: UniProt accession P20226 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 6908 Human
  • Entrez Gene: 21374 Mouse
  • Omim: 600075 Human
  • SwissProt: P20226 Human
  • SwissProt: P29037 Mouse
  • Unigene: 1100 Human
  • Unigene: 590872 Human
  • Unigene: 244820 Mouse

• Alternative names
  GTF2D antibodyGTF2D1 antibodyHDL4 antibody

  MGC117320 antibodyMGC126054 antibodyMGC126055 antibodySCA17 antibodyTATA binding factor antibodyTATA box factor antibodyTATA sequence binding protein antibodyTATA sequence-binding protein antibodyTATA-binding factor antibodyTATA-box binding protein N-terminal domain antibodyTATA-box factor antibodyTATA-box-binding protein antibodyTBP antibodyTBP_HUMAN antibodyTFIID antibodyTranscription initiation factor TFIID TBP subunit antibody

  see all

Anti-TATA binding protein TBP antibody - ChIP Grade images

• 

  ChIP - TATA binding protein TBP antibody (ab28175)

  Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The  ChIP was performed with 25µg of chromatin, 8µg of  ab28175 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman and sybr green approach). Primers and probes are located either in the core promoter of the gene (TATA) or in the open reading frame (ORF).