Anti-Calsequestrin antibody [VIIID12] (ab2824)

Overview

• Product nameAnti-Calsequestrin antibody [VIIID12]See all Calsequestrin primary antibodies ...
• Description
  Mouse monoclonal [VIIID12] to Calsequestrin
• SpecificityThis antibody recognizes calsequestrin in both type I (slow) and type II (fast) skeletal muscle tissues.
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Mouse, Rat, Rabbit, Dog
  
• Immunogen

  Purified rabbit skeletal muscle sarcoplasmic reticulum.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPBS with 0.05% sodium azide
• Concentration information loading...
• PurityAscites
• Clonality Monoclonal
• Clone numberVIIID12
• IsotypeIgG2b
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Signal Transduction
  • Signaling Pathway
  • Calcium Signaling
  • Calcium Binding Proteins

  • Signal Transduction
  • Protein Trafficking
  • ER Proteins

  • Cardiovascular
  • Heart
  • Cardiac metabolism

Applications

Target

• FunctionCalsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle. The release of calcium bound to calsequestrin through a calcium release channel triggers muscle contraction. Binds 40 to 50 moles of calcium. Also binds laminin.
• Sequence similaritiesBelongs to the calsequestrin family.
• Cellular localizationSarcoplasmic reticulum lumen. Mitochondrion. This isoform of calsequestrin occurs in the sarcoplasmic reticulum's terminal cisternae luminal spaces of fast skeletal muscle cells. Also mitochondrial according to PubMed:7945294.

Target information above from: UniProt accession P31415 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 445005 Dog
  • Entrez Gene: 12373 Mouse
  • Entrez Gene: 12372 Mouse
  • Entrez Gene: 29209 Rat
  • SwissProt: P12637 Dog
  • SwissProt: O09161 Mouse
  • SwissProt: O09165 Mouse
  • SwissProt: P31235 Rabbit

  • SwissProt: P07221 Rabbit
  • SwissProt: P51868 Rat
  • SwissProt: P19633 Rat
  • Unigene: 12829 Mouse

  see all

• Alternative names
  Calmitin antibodyCalsequestrin 1 antibodyCalsequestrin 1 fast twitch skeletal muscle antibody

  Calsequestrin 2 antibodyCalsequestrin 2 fast twitch cardiac muscle antibodyCalsequestrin antibodyCalsequestrin cardiac muscle isoform antibodyCalsequestrin skeletal muscle isoform antibodyCalsequestrin-1 antibodyCardiac calsequestrin 2 antibodyCardiac calsequestrin antibodyCASQ 1 antibodyCASQ 2 antibodyCASQ1 antibodyCASQ1_HUMAN antibodyCASQ2 antibodyCSQ1 antibodyFLJ26321 antibodyPDIB1 antibodyPDIB2 antibodySkeletal muscle calsequestrin 1 antibodyskeletal muscle isoform antibody

  see all

Anti-Calsequestrin antibody [VIIID12] images

• 

  Western blot - Calsequestrin antibody [VIIID12] (ab2824)
  Western blot of calsequestrin in canine skeletal muscle extract using ab2824.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Calsequestrin antibody [VIIID12](ab2824)
  Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human skeletal muscle tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing Calsequestrin ab2824 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Calsequestrin antibody [VIIID12](ab2824)
  Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human heart tissue tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Calsequestrin ab2824 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.