Anti-alpha 3 Sodium Potassium ATPase antibody [XVIF9-G10] (ab2826)

Overview

• Product nameAnti-alpha 3 Sodium Potassium ATPase antibody [XVIF9-G10]See all alpha 3 Sodium Potassium ATPase primary antibodies ...
• Description
  Mouse monoclonal [XVIF9-G10] to alpha 3 Sodium Potassium ATPase
• SpecificityDetects sodium / potassium ATPase. This antibody is specific for the alpha-3 subunit.
• Tested applicationsFlow Cyt, IHC-Fr, ICC/IF, WB more details
• Species reactivity
  Reacts with: Mouse, Rat, Sheep, Rabbit, Guinea pig, Dog, Human
  Predicted to work with: Non Human Primates
• Immunogen

  Canine cardiac microsomes

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPBS with 0.05% sodium azide
• Concentration information loading...
• PurityAscites
• Clonality Monoclonal
• Clone numberXVIF9-G10
• IsotypeIgG1
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Signal Transduction
  • Metabolism
  • Plasma Membrane
  • ATPases

  • Neuroscience
  • Neurotransmission
  • Receptors / Channels
  • Potassium Channels

  • Neuroscience
  • Neurotransmission
  • Receptors / Channels
  • Sodium Channels

Applications

Target

• FunctionThis is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
• Involvement in diseaseDefects in ATP1A3 are the cause of dystonia type 12 (DYT12) [MIM:128235]; also known as rapid-onset dystonia parkinsonism (RDP). DYT12 is an autosomal dominant dystonia-parkinsonism disorder. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. DYT12 patients develop dystonia and parkinsonism between 15 and 45 years of age. The disease is characterized by an unusually rapid evolution of signs and symptoms. The sudden onset of symptoms over hours to a few weeks, often associated with physical or emotional stress, suggests a trigger initiating a nervous system insult resulting in permanent neurologic disability.
  Defects in ATP1A3 are the cause of alternating hemiplegia of childhood 2 (AHC2) [MIM:614820]. A rare syndrome of episodic hemi- or quadriplegia lasting minutes to days. Most cases are accompanied by dystonic posturing, choreoathetoid movements, nystagmus, other ocular motor abnormalities, autonomic disturbances, and progressive cognitive impairment. It is typically distinguished from familial hemiplegic migraine by infantile onset and high prevalence of associated neurological deficits that become increasingly obvious with age.
• Sequence similaritiesBelongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.
• Cellular localizationCell membrane.

Target information above from: UniProt accession P13637 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 478 Human
  • Entrez Gene: 232975 Mouse
  • Entrez Gene: 24213 Rat
  • Omim: 182350 Human
  • SwissProt: P13637 Human
  • SwissProt: Q6PIC6 Mouse
  • SwissProt: P06687 Rat
  • Unigene: 515427 Human

  • Unigene: 44101 Mouse
  • Unigene: 87329 Rat

  see all

• Alternative names
  Alpha(III) antibodyAT1A3_HUMAN antibodyATP1A3 antibody

  ATPase Na+/K+ transporting alpha 3 polypeptide antibodyDYT 12 antibodyDYT12 antibodyMGC13276 antibodyNa(+)/K(+) ATPase alpha(III) subunit antibodyNa(+)/K(+) ATPase alpha-3 subunit antibodyNa+/K+ ATPase 3 antibodyNa+/K+ ATPase alpha 3 subunit antibodyRDP antibodySodium potassium ATPase alpha 3 polypeptide antibodySodium pump 3 antibodySodium pump subunit alpha-3 antibodySodium/potassium transporting ATPase alpha 3 chain antibodySodium/potassium-transporting ATPase subunit alpha-3 antibody

  see all

Anti-alpha 3 Sodium Potassium ATPase antibody [XVIF9-G10] images

• 

  Western blot - alpha 3 Sodium Potassium ATPase antibody [XVIF9-G10] (ab2826)
  Anti-alpha 3 Sodium Potassium ATPase antibody [XVIF9-G10] (ab2826) at 1/1000 dilution (5% Milk for 1 hour 30 minutes at 24°C.) + Human brain cortex whole tissue lysate (10µg). with 5% Milk for 1 hour for 24°C.
  
  Secondary
  An HRP-conjugated goat anti-mouse IgG1 at 1/5000 dilution

  This image is courtesy of an anonymous Abreview

  See Abreview

• 

  Flow Cytometry - Anti-alpha 3 Sodium Potassium ATPase antibody [XVIF9-G10] (ab2826)
  Overlay histogram showing SH-SY5Y cells stained with ab2826 (red line). The cells were fixed with 4% paraformaldehyde and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2826, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.
  
  Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-alpha 3 Sodium Potassium ATPase antibody [XVIF9-G10](ab2826)
  Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human prostate carcinoma tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:50 with a mouse monoclonal antibody recognizing Sodium/Potassium ATPase alpha-3 ab2826 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-alpha 3 Sodium Potassium ATPase antibody [XVIF9-G10](ab2826)
  Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human colon tissue tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Sodium/Potassium ATPase alpha-3 ab2826 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-alpha 3 Sodium Potassium ATPase antibody [XVIF9-G10](ab2826)
  Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human tonsil tissue tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Sodium/Potassium ATPase alpha-3 ab2826 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  
  
• 

  Immunocytochemistry/ Immunofluorescence-Anti-alpha 3 Sodium Potassium ATPase antibody [XVIF9-G10](ab2826)
  Immunofluorescent analysis of Sodium/Potassium ATPase alpha-3 using Sodium/Potassium ATPase alpha-3 Monoclonal antibody (XVIF9-G10) ab2826 shows staining in C6 glioma cells. Sodium/Potassium ATPase alpha-3 staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Sodium/Potassium ATPase alpha-3 ab2826 at a dilution of 1:20 over night at 4 ?C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
  
  
• 

  Immunocytochemistry/ Immunofluorescence-Anti-alpha 3 Sodium Potassium ATPase antibody [XVIF9-G10](ab2826)
  Immunofluorescent analysis of Sodium/Potassium ATPase alpha-3 using Sodium/Potassium ATPase alpha-3 Monoclonal antibody (XVIF9-G10) ab2826 shows staining in U251 glioma cells. Sodium/Potassium ATPase alpha-3 staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Sodium/Potassium ATPase alpha-3 ab2826 at a dilution of 1:20 over night at 4 ?C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
  
  
• 

  Immunocytochemistry/ Immunofluorescence-Anti-alpha 3 Sodium Potassium ATPase antibody [XVIF9-G10](ab2826)
  Immunofluorescent analysis of Sodium/Potassium ATPase alpha-3 using Sodium/Potassium ATPase alpha-3 Monoclonal antibody (XVIF9-G10) ab2826 shows staining in HeLa cells. Sodium/Potassium ATPase alpha-3 staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Sodium/Potassium ATPase alpha-3 ab2826 at a dilution of 1:20 over night at 4 ?C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.