Anti-ADAMTS1 antibody - Aminoterminal end (ab28284)
- Product nameAnti-ADAMTS1 antibody - Aminoterminal endSee all ADAMTS1 primary antibodies ...
- DescriptionRabbit polyclonal to ADAMTS1 - Aminoterminal end
- SpecificityThis antibody recognizes the zymogen and active forms of ADAMTS 1.
- Tested applicationsIHC-P, Neutralising, WB, ICC/IF more details
- Species reactivityReacts with: Human, Rhesus monkey
Synthetic peptide based on the aminoterminal end of furin cleaved human ADAMTS-1.
(Peptide available as ab41230.)
- EpitopeThis antibody recognizes the aminoterminal end of furin cleaved ADAMTS-1, after the second furin cleavage site.
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
- Concentration information loading...
- PurityImmunogen affinity purified
- Purification notesThis antibody has been peptide-affinity purified.
- Clonality Polyclonal
Our Abpromise guarantee covers the use of ab28284 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||IHC-P: Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|Neutralising||Neut: Use at an assay dependent dilution. PubMed: 20840749|
|WB||WB: 1/1000 - 1/5000. Detects a band of approximately 110-120, 84-98 kDa (predicted molecular weight: 106 kDa).Can be blocked with ADAMTS1 peptide (Aminoterminal end) (ab41230). Detects bands of approximately 110-120 (due to glycoslylation and cysteine rich regions of zymogen form) and a 84-98 kDa band, (active form, major band). (Predicted molecular weight: 106 kDa). Note: 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen.|
|ICC/IF||ICC/IF: Use a concentration of 1 µg/ml.|
- FunctionCleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover (By similarity). Has angiogenic inhibitor activity. Active metalloprotease, which may be associated with various inflammatory processes as well as development of cancer cachexia. May play a critical role in follicular rupture.
- Sequence similaritiesContains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
Contains 3 TSP type-1 domains.
- DomainThe spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
modificationsThe precursor is cleaved by a furin endopeptidase.
- Cellular localizationSecreted > extracellular space > extracellular matrix.
- A disintegrin and metalloproteinase with thrombospondin motifs 1 antibodyA disintegrin like and metalloprotease with thrombospondin type 1 motif 1 antibodyA disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 1 antibody
- A disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 1 antibodyADAM metallopeptidase with thrombospondin type 1 motif 1 antibodyADAM TS 1 antibodyADAM TS1 antibodyADAM-TS 1 antibodyADAM-TS1 antibodyADAMTS 1 antibodyADAMTS antibodyADAMTS-1 antibodyAdamts1 antibodyATS1_HUMAN antibodyC3 C5 antibodyKIAA1346 antibodyKIAA1346, antibodyMETH 1 antibodyMETH-1 antibodyMETH1 antibodyMETH1 antibody
Anti-ADAMTS1 antibody - Aminoterminal end images
Anti-ADAMTS1 antibody - Aminoterminal end (ab28284) at 1/2500 dilution + SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 106 kDa
Observed band size : 110 kDa (why is the actual band size different from the predicted?)
Additional bands at : 43 kDa. We are unsure as to the identity of these extra bands.
ICC/IF image of ab28284 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28284, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
IHC image of ab28284 staining in human liver carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab28284, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-ADAMTS1 antibody - Aminoterminal end (ab28284)
This product has been referenced in:
- Peluffo MC et al. Systematic analysis of protease gene expression in the rhesus macaque ovulatory follicle: metalloproteinase involvement in follicle rupture. Endocrinology 152:3963-74 (2011). IHC-P ; Rhesus monkey . Read more (PubMed: 21791558) »
- Keightley MC et al. PGF2a-F-prostanoid receptor signalling via ADAMTS1 modulates epithelial cell invasion and endothelial cell function in endometrial cancer. BMC Cancer 10:488 (2010). IHC-P, Neutralising ; Human . Read more (PubMed: 20840749) »