Anti-ADAMTS1 antibody - Aminoterminal end (ab28284)

Overview

• Product nameAnti-ADAMTS1 antibody - Aminoterminal endSee all ADAMTS1 primary antibodies ...
• Description
  Rabbit polyclonal to ADAMTS1 - Aminoterminal end
• SpecificityThis antibody recognizes the zymogen and active forms of ADAMTS 1.
• Tested applicationsIHC-P, Neutralising, WB, ICC/IF more details
• Species reactivity
  Reacts with: Human, Rhesus monkey
  
• Immunogen

  Synthetic peptide based on the aminoterminal end of furin cleaved human ADAMTS-1.

  Read Abcam's proprietary immunogen policy

  (Peptide available as ab41230.)

• EpitopeThis antibody recognizes the aminoterminal end of furin cleaved ADAMTS-1, after the second furin cleavage site.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.05% Sodium Azide
  Constituents: 50% Glycerol
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesThis antibody has been peptide-affinity purified.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Invasion/microenvironment
  • ECM
  • Extracellular matrix
  • ADAM protein family

  • Signal Transduction
  • Cytoskeleton / ECM
  • Extracellular Matrix
  • ECM Enzymes
  • MMP

  • Cardiovascular
  • Angiogenesis
  • Adhesion / ECM
  • Matrix Metalloproteinases
  • MMP

  • Tags & Cell Markers
  • Cell Type Markers
  • Tumor Associated

  • Cell Biology
  • Proteolysis / Ubiquitin
  • Proteolytic enzymes
  • Metalloprotease
  • ADAM TS

Applications

Target

• FunctionCleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover (By similarity). Has angiogenic inhibitor activity. Active metalloprotease, which may be associated with various inflammatory processes as well as development of cancer cachexia. May play a critical role in follicular rupture.
• Sequence similaritiesContains 1 disintegrin domain.
  Contains 1 peptidase M12B domain.
  Contains 3 TSP type-1 domains.
• DomainThe spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
  The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
• Post-translational
  modificationsThe precursor is cleaved by a furin endopeptidase.
• Cellular localizationSecreted > extracellular space > extracellular matrix.

Target information above from: UniProt accession Q9UHI8 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 9510 Human
  • Entrez Gene: 9510 Human
  • Omim: 605174 Human
  • SwissProt: Q9UHI8 Human
  • SwissProt: Q9UHI8 Human
  • Unigene: 8230 Human
  • Unigene: 643357 Human

• Alternative names
  A disintegrin and metalloproteinase with thrombospondin motifs 1 antibodyA disintegrin like and metalloprotease with thrombospondin type 1 motif 1 antibodyA disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 1 antibody

  A disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 1 antibodyADAM metallopeptidase with thrombospondin type 1 motif 1 antibodyADAM TS 1 antibodyADAM TS1 antibodyADAM-TS 1 antibodyADAM-TS1 antibodyADAMTS 1 antibodyADAMTS antibodyADAMTS-1 antibodyAdamts1 antibodyATS1_HUMAN antibodyC3 C5 antibodyKIAA1346 antibodyKIAA1346, antibodyMETH 1 antibodyMETH-1 antibodyMETH1 antibodyMETH1 antibody

  see all

Anti-ADAMTS1 antibody - Aminoterminal end images

• 

  Western blot - ADAMTS1 antibody - Aminoterminal end (ab28284)
  Anti-ADAMTS1 antibody - Aminoterminal end (ab28284) at 1/2500 dilution + SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate at 10 µg
  
  Secondary
  Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  
  Predicted band size : 106 kDa
  Observed band size : 110 kDa (why is the actual band size different from the predicted?)
  Additional bands at : 43 kDa. We are unsure as to the identity of these extra bands.
• 

  Immunocytochemistry/ Immunofluorescence - Anti-ADAMTS1 antibody - Aminoterminal end (ab28284)
  ICC/IF image of ab28284 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28284, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAMTS1 antibody - Aminoterminal end (ab28284)
  IHC image of ab28284 staining in human liver carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab28284, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  
  

Protocols

• Recommended WB protocol