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Anti-ADAM20 antibody - Catalytic domain (ab28295)

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Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab28295 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
  • Application notesWB: A recommended starting concentration for Western Blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Predicted molecular weight: 82 kDa. Higher concentrations of antibody may be needed for samples from more distantly related species. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. This product recognizes bands of 95 kDa, 84 kDa (major band), and breakdown products at 50 kDa, 34 kDa in reduced Western Blots of cell lysates. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionMay be involved in sperm maturation and/or fertilization.
    • Tissue specificityTestis specific.
    • Sequence similaritiesContains 1 disintegrin domain.
      Contains 1 EGF-like domain.
      Contains 1 peptidase M12B domain.
    • DomainA tripeptide motif (VGE) within disintegrin-like domain could be involved in the binding to egg integrin receptor and thus could mediate sperm/egg binding.
      The cysteine-rich domain encodes putative cell-fusion peptides, which could be involved in sperm-egg fusion.
      The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • Post-translational
      modifications      Has no obvious cleavage site for furin endopeptidase, suggesting that the proteolytic processing is regulated.
    • Cellular localizationMembrane.

      • Target information above from: UniProt accession O43506 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      • Alternative names
          A disintegrin and metalloproteinase domain 20 antibodyA disintegrin and metalloproteinase domain 20 preproprotein antibodyADA20_HUMAN antibody
          ADAM 20 antibodyADAM metallopeptidase domain 20 antibodyADAM20 antibodyDisintegrin and metalloproteinase domain-containing protein 20 antibodyEC 3.4.24. antibodyOTTHUMP00000236151 antibody
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      Anti-ADAM20 antibody - Catalytic domain images

      • Western blot - ADAM20 antibody - Catalytic domain (ab28295)
        Western blot - ADAM20 antibody - Catalytic domain (ab28295)
        All lanes : Anti-ADAM20 antibody - Catalytic domain (ab28295) at 1/1000 dilution

        Lane 1 : Mouse Tetis Lysate
        Lane 2 : Mouse Epididymis Lysate

        Lysates/proteins at 20 µl per lane.


        Predicted band size : 82 kDa

      References for Anti-ADAM20 antibody - Catalytic domain (ab28295)

      ab28295 has not yet been referenced specifically in any publications.

      Publishing research using ab28295 ? Please let us know so that we can cite the reference in this datasheet

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      What is the peptide sequences recognised by : ab28468, ab28290, ab28288,ab38944, ab39879, ab40136, ab28295, ab40132, ab28293 ?

      Dear Cecil In response to your query, I'm afraid I can't provide you with more specific details on the peptide sequence because these amino acid sequences are proprietary. The only information I can provide you with is the following details: # ...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"