Overview
- Product nameAnti-l-Myc antibodySee all l-Myc primary antibodies ...
- DescriptionRabbit polyclonal to l-Myc
- Tested applicationsIHC-P, WB more details
- Species reactivityReacts with: Mouse, Human
Predicted to work with: Dog - Immunogen
A region within synthetic peptide: LERAVSDRLA PGAPRGNPPK ASAAPDCTPS LEAGNPAPAA PCPLGEPKTQ, corresponding to internal sequence amino acids 105-154 of Human l-Myc
- Positive controlHuman foetal kidney lysate, human kidney tissue.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferLyophilised from -
Preservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... - PurityProtein A purified
- Clonality Polyclonal
- IsotypeIgG
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Research Areas
Applications
Our Abpromise guarantee covers the use of ab28739 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| IHC-P | IHC-P: Use a concentration of 4 - 8 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
| WB | WB: Use a concentration of 2.5 µg/ml. Predicted molecular weight: 40,25 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. |
Target
- Sequence similaritiesContains 1 bHLH (basic helix-loop-helix) domain.
- Cellular localizationNucleus.
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Database links
- Entrez Gene: 4610 Human
- Entrez Gene: 4610 Human
- Entrez Gene: 16918 Mouse
- Omim: 164850 Human
- SwissProt: P12524 Human
- SwissProt: P12524 Human
- SwissProt: P10166 Mouse
- Unigene: 437922 Human
- Unigene: 1055 Mouse
- Unigene: 479066 Mouse
see all
Target information above from: UniProt accession
P12524
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- bHLHe38 antibodyClass E basic helix-loop-helix protein 38 antibodyl myc 1 proto oncogene antibody
- L myc 1 proto-oncogene protein antibodyl myc protein antibodyLMYC antibodyMyc related gene from lung cancer antibodyMYCL 1 antibodyMYCL antibodyMYCL1 antibodyMYCL1_HUMAN antibodyoncogene lmyc antibodyProtein L-Myc-1 antibodyv myc avian myelocytomatosis viral oncogene homolog 1 lung carcinoma derived antibody
see all
Anti-l-Myc antibody images
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Immunohistochemistry (Paraffin-embedded sections) - l-Myc antibody (ab28739)Immunohistochemical staining of paraffin embedded human kidney with ab28739 at 4-8 ug/ml. Arrows indicate postive staining of epithelial cells of the renal tube. -
Western blot - l-Myc antibody (ab28739)Anti-l-Myc antibody (ab28739) at 2.5 µg/ml + Human foetal kidney lysate
Secondary
HRP conjugated anti-Rabbit IgG 1: 50,000 - 100,000
Predicted band size : 40,25 kDa
Observed band size : 23 kDa (why is the actual band size different from the predicted?)
References for Anti-l-Myc antibody (ab28739)
ab28739 has not yet been referenced specifically in any publications.
