Anti-Cytochrome b reductase 1 antibody (ab28758)

Overview

• Product nameAnti-Cytochrome b reductase 1 antibodySee all Cytochrome b reductase 1 primary antibodies ...
• Description
  Goat polyclonal to Cytochrome b reductase 1
• Tested applicationsICC/IF, IHC-Fr, IHC-FoFr, IHC-P more details
• Species reactivity
  Reacts with: Mouse, Human
  Predicted to work with: Cow, Dog, Pig
• Immunogen

  Synthetic peptide: (C)RNLALDEAGQRSTM, corresponding to C terminal amino acids 273-286 of Human Cytochrome b reductase 1

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Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 0.5% BSA, 5mg/ml Tris, pH 7.3
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesThis antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Cofactors, Vitamins / minerals
  • Vitamins / minerals

  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Cytochromes

  • Signal Transduction
  • Metabolism
  • Vitamins / Minerals

Applications

Target

• FunctionFerric-chelate reductase that reduces Fe(3+) to Fe(2+). Present at the brush border of duodenal enterocytes where it probably reduces dietary Fe(3+) thereby facilitating its transport into the mucosal cells. Uses ascorbate as electron donor. May be involved in extracellular ascorbate recycling in erythrocyte membranes. May also act as a ferrireductase in airway epithelial cells.
• Tissue specificityPresent in erythrocyte membranes (at protein level). Also expressed in respiratory epithelium.
• Sequence similaritiesContains 1 cytochrome b561 domain.
• Cellular localizationMembrane.

Target information above from: UniProt accession Q53TN4 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 79901 Human
  • Entrez Gene: 73649 Mouse
  • Omim: 605745 Human
  • SwissProt: Q53TN4 Human
  • SwissProt: Q925G2 Mouse
  • Unigene: 741213 Human
  • Unigene: 726027 Human
  • Unigene: 45435 Mouse

• Alternative names
  CYBR 1 antibodyCYBR1 antibodyCYBR1_HUMAN antibody

  CYBRD 1 antibodyCYBRD1 antibodyCytochrome b reductase 1 antibodyDCYTB antibodyDKFZp564E227 antibodyDuodenal cytochrome b antibodyFerric chelate reductase 3 antibodyFerric-chelate reductase 3 antibodyFLJ23462 antibodyFRRS3 antibody

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Anti-Cytochrome b reductase 1 antibody images

• 

  Immunohistochemistry (PFA perfusion fixed frozen sections) - Cytochrome b reductase 1 antibody (ab28758)Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom
  ICC/IF image of adult mouse gut section stained with (ab28758) . Tissue was perfusion fixed with 4% PFA. The sections were incubated in 10% normal donkey serum in 0.1% PBS- and triton X100 for 1h to permeabilise the cells and block non-specific protein-protein interactions. The sections were then incubated with the antibody (ab28758) @ 1µg/ml) overnight @ 4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

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• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome b reductase 1 antibody (ab28758)
  ab28758 at 2.5 µg/ml staining Cytochrome b reductase 1 in Human testis by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
• 

  Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome b reductase 1 antibody (ab28758)Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom
  ICC/IF image of Cytochrome b reductase staining on Caco 2 cells using ab28758 (1:200). The cells were fixed using 4% PFA and permeabilized using 0.1% TritonX in 0.1% PBS. The cells were blocked by incubating 10% donkey serum for 1 hour at 24°C. ab28758 (1:200) was incubated for 4 hours at 24°C. the secondary antibody used was donkey polyclonal to Goat IgG conjugated to Alexa Fluor 568.

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