Anti-ATF2 (phospho T69 + T51) antibody (ab28848)
Overview
- Product nameAnti-ATF2 (phospho T69 + T51) antibodySee all ATF2 primary antibodies ...
- DescriptionRabbit polyclonal to ATF2 (phospho T69 + T51)
- Tested applicationsELISA, IHC-P, IP, WB more details
- Species reactivityReacts with: Mouse, Rat, Human
- Immunogen
Synthetic phosphopeptide derived from human ATF2 around the phosphorylation site of Threonine 69/51.
- Positive controlWB: LOVO cell lysate. IHC-P: breast carcinoma.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide.
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4. -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesThe antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab28848 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ELISA | ELISA: 1/20000. |
| IHC-P | IHC-P: Use at an assay dependent dilution. |
| IP | IP: Use at an assay dependent dilution. |
| WB | WB: 1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). |
Target
- FunctionTranscriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex.
- Tissue specificityAbundant expression seen in the brain.
- Sequence similaritiesBelongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain.
Contains 1 C2H2-type zinc finger. - Post-translational
modificationsPhosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK. - Cellular localizationNucleus.
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Database links
- Entrez Gene: 1386 Human
- Entrez Gene: 100047997 Mouse
- Entrez Gene: 11909 Mouse
- Entrez Gene: 81647 Rat
- Omim: 123811 Human
- SwissProt: P15336 Human
- SwissProt: P16951 Mouse
- SwissProt: Q00969 Rat
- Unigene: 592510 Human
- Unigene: 209903 Mouse
- Unigene: 9825 Rat
see all
Target information above from: UniProt accession
P15336
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
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Alternative names
- Activating Transcription Factor 2 antibodyActivating transcription factor 2 splice variant ATF2 var2 antibodyATF 2 antibody
- Atf-2 antibodyAtf2 antibodyATF2 protein antibodyATF2_HUMAN antibodycAMP Response Element Binding Protein 2 antibodycAMP response element binding protein CRE BP1 antibodycAMP response element-binding protein CRE-BP1 antibodycAMP responsive element binding protein 2, formerly antibodycAMP-dependent transcription factor ATF-2 antibodycAMP-responsive element-binding protein 2 antibodyCRE BP1 antibodyCRE-BP antibodyCREB 2 antibodyCREB 2 antibodyCREB-2 antibodyCREB2 antibodyCREBP1 antibodyCyclic AMP dependent transcription factor ATF 2 antibodyCyclic AMP-dependent transcription factor ATF-2 antibodyCyclic AMP-responsive element-binding protein 2 antibodyD130078H02Rik antibodyD18875 antibodyHB 16 antibodyHB 16 antibodyHB16 antibodyMGC105211 antibodyMGC105222 antibodyMGC111558 antibodyMGC142504 antibodymXBP antibodyMXBP protein antibodyTg(Gzma-Klra1)7Wum antibodyTREB 7 antibodyTREB7 antibody
see all
Anti-ATF2 (phospho T69 + T51) antibody images
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Immunohistochemistry (Paraffin-embedded sections) - ATF2 (phospho T69/51) antibody (ab28848)Ab28848, at a dilution of 1/50, staining ATF2 in paraffin embedded breast carcinoma by Immunohistochemistry.
Left image: using ab28848, phosphorylated ATF2 (Thr69/51) antibody.
Right image: the same antibody preincubated with synthesized phosphopeptide. -
Western blot - ATF2 (phospho T69/51) antibody (ab28848)All lanes : Anti-ATF2 (phospho T69 + T51) antibody (ab28848)
Lane 1 : LOVO cell lysate (5-30ug).
Lane 2 : LOVO cell lysate (5-30ug). with Immunizing peptide
Predicted band size : 52 kDa
Observed band size : 52 kDa -
Western blot - ATF2 (phospho T69/51) antibody (ab28848)Anti-ATF2 (phospho T69 + T51) antibody (ab28848) at 1/750 dilution
Secondary
HRP conjugated sheep anti-rabbit IgG polyclonal at 1/20000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 52 kDa
Observed band size : 52 kDa
Additional bands at : 47 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 4 minutesThis image is courtesy of an anonymous Abreview
References for Anti-ATF2 (phospho T69 + T51) antibody (ab28848)
This product has been referenced in:
- Diring J et al. A Cytoplasmic Negative Regulator Isoform of ATF7 Impairs ATF7 and ATF2 Phosphorylation and Transcriptional Activity. PLoS One 6:e23351 (2011). WB ; Human . Read more (PubMed: 21858082) »
