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Overview

  • Product nameAnti-Dnmt3L antibodySee all Dnmt3L primary antibodies ...
  • Description
    Chicken polyclonal to Dnmt3L
  • Specificityab28968 recognises Dnmt3L.
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified recombinant full length Dnmt3L protein (Human)

  • Positive controlPurified recombinant Human Dnmt3L.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purification notesab28968 is a crude IgY preparation from egg yolks using a precipitation method (PEG, Polyethylene Glycol).
  • Clonality Polyclonal
  • IsotypeIgY
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab28968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/500 - 1/5000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).

Target

  • FunctionCatalytically inactive regulatory factor of DNA methyltransferases. It is essential for the function of DNMT3A and DNMT3B. Activates DNMT3A and DNMT3B by binding to their catalytic domain. Accelerates the binding of DNA and AdoMet to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases. Recognizes unmethylated histone H3 lysine 4 (H3K4) and induces de novo DNA methylation by recruitment or activation of DNMT3.
  • Tissue specificityExpressed at low levels in several tissues including testis, ovary, and thymus.
  • Sequence similaritiesBelongs to the C5-methyltransferase family.
    Contains 1 ADD-type zinc finger.
  • Cellular localizationNucleus.

    • Target information above from: UniProt accession Q9UJW3 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        Cytosine 5 methyltransferase 3 like protein antibodyDNA (cytosine 5 ) methyltransferase 3 like antibodyDNA (cytosine-5)-methyltransferase 3-like antibody
        DNA Cytosine 5 Methyltransferase 3 Like Protein antibodyDNA Cytosine 5 Methyltransferase 3 Like Protein antibodyDNA Methyltransferase 3 Like Protein antibodyDNA Methyltransferase 3 Like Protein antibodyDNM3L_HUMAN antibodyDnmt 3L antibodyDnmt3l antibodyHuman cytosine 5 methyltransferase 3 like protein antibodyMGC1090 antibody
      see all

    Anti-Dnmt3L antibody images

    • Western blot - Dnmt3L antibody (ab28968)
      Western blot - Dnmt3L antibody (ab28968)
      Anti-Dnmt3L antibody (ab28968) at 1/500 dilution

      Predicted band size : 47 kDa
      Observed band size : 47 kDa

    References for Anti-Dnmt3L antibody (ab28968)

    ab28968 has not yet been referenced specifically in any publications.

    Publishing research using ab28968 ? Please let us know so that we can cite the reference in this datasheet

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    Western blot abreview for Anti-Dnmt3L antibody

     Average
    Abreviews
    Application Western blot
    Sample Human Cell lysate - nuclear (Hela cells)
    Loading amount 40 µg
    Specification Hela cells
    Gel Running Conditions Reduced Denaturing (12%)
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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    Submitted Sep 16 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"