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Liver (Mouse) Membrane Lysate - normal tissue (ab29299)

Q&A (2)

Overview

Properties

  • FormLiquid
  • Storage instructionsStore at -80°C. Allow to warm to room temp and agitate gently before aliquotting.
  • Storage bufferPreservative: None
    Constituents: 2% SDS, 10% Glycerol, 0.1% Bromophenol blue, Protease inhibitor, Sodium chloride, EDTA, Magnesium chloride, HEPES, 0.15M Beta mercaptoethanol
  • Concentration information loading...
  • Lysate notes Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot.
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab29299 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use at an assay dependent dilution. Samples need to boiled for 5 minutes and put on ice before use.

References for Liver (Mouse) Membrane Lysate - normal tissue (ab29299)

ab29299 has not yet been referenced specifically in any publications.

Publishing research using ab29299 ? Please let us know so that we can cite the reference in this datasheet

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Submit a Question for ab29299

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In the attachment, you can find the picture of the Western Blot. For this blot, we only loaded 13 µg of the samples, and here we see 3 bands for HTR4. I repeated the blot with 25 µg of sample and there we see an additional band between the second and the ...

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Thank you very much for confirming these details and sending the excellent image! The band at a different molecular weight detected in the liver sample (lane 2) may be due to a tissue-specific post-translational modification pattern: According to the l...

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LOT NUMBER -- NOT SPECIFIED -- ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM Multiple bands I see 4 bands in all samples: 2 dark ones, 1 very faint one and 1 light band. Which one is specific for HTR4? What are the other bands? SAMPLE murine...

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this 5HT4 antibody. Reviewing this case, I would appreciate if you can confirm some further deta...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"